PMID- 10205180
OWN - NLM
STAT- MEDLINE
DCOM- 19990611
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 8
DP  - 1999 Apr 15
TI  - Influenza A virus NS1 protein targets poly(A)-binding protein II of the cellular 
      3'-end processing machinery.
PG  - 2273-83
AB  - Influenza A virus NS1 protein (NS1A protein) via its effector domain targets the 
      poly(A)-binding protein II (PABII) of the cellular 3'-end processing machinery.
      In vitro the NS1A protein binds the PABII protein, and in vivo causes PABII
      protein molecules to relocalize from nuclear speckles to a uniform distribution
      throughout the nucleoplasm. In vitro the NS1A protein inhibits the ability of
      PABII to stimulate the processive synthesis of long poly(A) tails catalyzed by
      poly(A) polymerase (PAP). Such inhibition also occurs in vivo in influenza
      virus-infected cells, where the NS1A protein via its effector domain causes the
      nuclear accumulation of cellular pre-mRNAs which contain short ( approximately 12
      nucleotide) poly(A) tails. Consequently, although the NS1A protein also binds the
      30 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF), 3' 
      cleavage of some cellular pre-mRNAs still occurs in virus-infected cells,
      followed by the PAP-catalyzed addition of short poly(A) tails. Subsequent
      elongation of these short poly(A) tails is blocked because the NS1A protein
      inhibits PABII function. Nuclear-cytoplasmic shuttling of PABII, an activity
      implicating this protein in the nuclear export of cellular mRNAs, is also
      inhibited by the NS1A protein. In vitro assays suggest that the 30 kDa CPSF and
      PABII proteins bind to non-overlapping regions of the NS1A protein effector
      domain and indicate that these two 3' processing proteins also directly bind to
      each other.
FAU - Chen, Z
AU  - Chen Z
AD  - Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway,
      NJ 08855, USA.
FAU - Li, Y
AU  - Li Y
FAU - Krug, R M
AU  - Krug RM
LA  - eng
GR  - AI11772/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA Primers)
RN  - 0 (INS1 protein, influenza virus)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Base Sequence
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - DNA Primers
MH  - Fluorescent Antibody Technique, Indirect
MH  - Influenza A virus/metabolism
MH  - Microscopy, Confocal
MH  - Poly(A)-Binding Proteins
MH  - Protein Binding
MH  - *Protein Processing, Post-Translational
MH  - RNA-Binding Proteins/*metabolism
MH  - Viral Nonstructural Proteins/*metabolism
PMC - PMC1171310
EDAT- 1999/04/16 00:00
MHDA- 1999/04/16 00:01
CRDT- 1999/04/16 00:00
PHST- 1999/04/16 00:00 [pubmed]
PHST- 1999/04/16 00:01 [medline]
PHST- 1999/04/16 00:00 [entrez]
AID - 10.1093/emboj/18.8.2273 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 15;18(8):2273-83. doi: 10.1093/emboj/18.8.2273.