PMID- 10205173
OWN - NLM
STAT- MEDLINE
DCOM- 19990611
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 8
DP  - 1999 Apr 15
TI  - Hes1 and Hes5 as notch effectors in mammalian neuronal differentiation.
PG  - 2196-207
AB  - While the transmembrane protein Notch plays an important role in various aspects 
      of development, and diseases including tumors and neurological disorders, the
      intracellular pathway of mammalian Notch remains very elusive. To understand the 
      intracellular pathway of mammalian Notch, the role of the bHLH genes Hes1 and
      Hes5 (mammalian hairy and Enhancer-of-split homologues) was examined by
      retrovirally misexpressing the constitutively active form of Notch (caNotch) in
      neural precursor cells prepared from wild-type, Hes1-null, Hes5-null and
      Hes1-Hes5 double-null mouse embryos. We found that caNotch, which induced the
      endogenous Hes1 and Hes5 expression, inhibited neuronal differentiation in the
      wild-type, Hes1-null and Hes5-null background, but not in the Hes1-Hes5
      double-null background. These results demonstrate that Hes1 and Hes5 are
      essential Notch effectors in regulation of mammalian neuronal differentiation.
FAU - Ohtsuka, T
AU  - Ohtsuka T
AD  - Institute for Virus Research, Kyoto University, Shogoin-Kawahara, Sakyo-ku, Kyoto
      606-8507, USA.
FAU - Ishibashi, M
AU  - Ishibashi M
FAU - Gradwohl, G
AU  - Gradwohl G
FAU - Nakanishi, S
AU  - Nakanishi S
FAU - Guillemot, F
AU  - Guillemot F
FAU - Kageyama, R
AU  - Kageyama R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Hes1 protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Receptors, Notch)
RN  - 0 (Transcription Factor HES-1)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Gene Expression Regulation/genetics
MH  - *Homeodomain Proteins
MH  - Immunohistochemistry
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Muscle Proteins/*physiology
MH  - Neurons/*cytology
MH  - Receptors, Notch
MH  - Retroviridae/genetics
MH  - Transcription Factor HES-1
PMC - PMC1171303
EDAT- 1999/04/16 00:00
MHDA- 1999/04/16 00:01
CRDT- 1999/04/16 00:00
PHST- 1999/04/16 00:00 [pubmed]
PHST- 1999/04/16 00:01 [medline]
PHST- 1999/04/16 00:00 [entrez]
AID - 10.1093/emboj/18.8.2196 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 15;18(8):2196-207. doi: 10.1093/emboj/18.8.2196.