PMID- 10205167
OWN - NLM
STAT- MEDLINE
DCOM- 19990611
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 8
DP  - 1999 Apr 15
TI  - Fetal liver development requires a paracrine action of oncostatin M through the
      gp130 signal transducer.
PG  - 2127-36
AB  - Fetal liver, the major site of hematopoiesis during embryonic development,
      acquires additional various metabolic functions near birth. Although liver
      development has been characterized biologically as consisting of several distinct
      steps, the molecular events accompanying this process are just beginning to be
      characterized. In this study, we have established a novel culture system of fetal
      murine hepatocytes and investigated factors required for development of
      hepatocytes. We found that oncostatin M (OSM), an interleukin-6 family cytokine, 
      in combination with glucocorticoid, induced maturation of hepatocytes as
      evidenced by morphological changes that closely resemble more differentiated
      hepatocytes, expression of hepatic differentiation markers and intracellular
      glycogen accumulation. Consistent with these in vitro observations, livers from
      mice deficient for gp130, an OSM receptor subunit, display defects in maturation 
      of hepatocytes. Interestingly, OSM is expressed in CD45(+) hematopoietic cells in
      the developing liver, whereas the OSM receptor is expressed predominantly in
      hepatocytes. These results suggest a paracrine mechanism of hepatogenesis; blood 
      cells, transiently expanding in the fetal liver, produce OSM to promote
      development of hepatocytes in vivo.
FAU - Kamiya, A
AU  - Kamiya A
AD  - Laboratory of Cellular Biosynthesis, Institute of Molecular and Cellular
      Biosciences, University of Tokyo, Bunkyo-ku, 113-0032 Tokyo.
FAU - Kinoshita, T
AU  - Kinoshita T
FAU - Ito, Y
AU  - Ito Y
FAU - Matsui, T
AU  - Matsui T
FAU - Morikawa, Y
AU  - Morikawa Y
FAU - Senba, E
AU  - Senba E
FAU - Nakashima, K
AU  - Nakashima K
FAU - Taga, T
AU  - Taga T
FAU - Yoshida, K
AU  - Yoshida K
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Miyajima, A
AU  - Miyajima A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Antigens, CD)
RN  - 0 (Il6st protein, mouse)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Osm protein, mouse)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Oncostatin M)
RN  - 106956-32-5 (Oncostatin M)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokine Receptor gp130
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Gluconeogenesis/drug effects
MH  - Liver/cytology/*embryology
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oncostatin M
MH  - Peptides/*pharmacology
MH  - Receptors, Cytokine/genetics
MH  - Receptors, Oncostatin M
PMC - PMC1171297
EDAT- 1999/04/16 00:00
MHDA- 1999/04/16 00:01
CRDT- 1999/04/16 00:00
PHST- 1999/04/16 00:00 [pubmed]
PHST- 1999/04/16 00:01 [medline]
PHST- 1999/04/16 00:00 [entrez]
AID - 10.1093/emboj/18.8.2127 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 15;18(8):2127-36. doi: 10.1093/emboj/18.8.2127.