PMID- 10205060
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 284
IP  - 5413
DP  - 1999 Apr 16
TI  - Control of mRNA decay by heat shock-ubiquitin-proteasome pathway.
PG  - 499-502
AB  - Cytokine and proto-oncogene messenger RNAs (mRNAs) are rapidly degraded through
      AU-rich elements in the 3' untranslated region. Rapid decay involves AU-rich
      binding protein AUF1, which complexes with heat shock proteins hsc70-hsp70,
      translation initiation factor eIF4G, and poly(A) binding protein. AU-rich mRNA
      decay is associated with displacement of eIF4G from AUF1, ubiquitination of AUF1,
      and degradation of AUF1 by proteasomes. Induction of hsp70 by heat shock,
      down-regulation of the ubiquitin-proteasome network, or inactivation of
      ubiquitinating enzyme E1 all result in hsp70 sequestration of AUF1 in the
      perinucleus-nucleus, and all three processes block decay of AU-rich mRNAs and
      AUF1 protein. These results link the rapid degradation of cytokine mRNAs to the
      ubiquitin-proteasome pathway.
FAU - Laroia, G
AU  - Laroia G
AD  - Department of Microbiology and Biochemistry, New York University School of
      Medicine, New York, NY 10016, USA.
FAU - Cuesta, R
AU  - Cuesta R
FAU - Brewer, G
AU  - Brewer G
FAU - Schneider, R J
AU  - Schneider RJ
LA  - eng
GR  - CA42357/CA/NCI NIH HHS/United States
GR  - CA52443/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Eukaryotic Initiation Factor-4G)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSPA8 protein, human)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein D)
RN  - 0 (Leupeptins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ubiquitins)
RN  - 0 (hnRNP D0)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Carrier Proteins/metabolism
MH  - Cell Nucleus/metabolism
MH  - Cysteine Endopeptidases/*metabolism
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Cytoplasm/metabolism
MH  - Eukaryotic Initiation Factor-4G
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*genetics
MH  - HSC70 Heat-Shock Proteins
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - HeLa Cells
MH  - *Heat-Shock Response
MH  - *Heterogeneous-Nuclear Ribonucleoprotein D
MH  - Humans
MH  - Leupeptins/pharmacology
MH  - Multienzyme Complexes/*metabolism
MH  - Peptide Initiation Factors/metabolism
MH  - Poly(A)-Binding Proteins
MH  - Proteasome Endopeptidase Complex
MH  - Protein Binding
MH  - RNA, Messenger/genetics/*metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Transfection
MH  - Ubiquitins/*metabolism
EDAT- 1999/04/16 00:00
MHDA- 1999/04/16 00:01
CRDT- 1999/04/16 00:00
PHST- 1999/04/16 00:00 [pubmed]
PHST- 1999/04/16 00:01 [medline]
PHST- 1999/04/16 00:00 [entrez]
AID - 10.1126/science.284.5413.499 [doi]
PST - ppublish
SO  - Science. 1999 Apr 16;284(5413):499-502. doi: 10.1126/science.284.5413.499.