PMID- 10202156
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190524
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Apr 1
TI  - Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode 
      centromere-associated proteins which complex with the heterochromatin component
      M31.
PG  - 1923-38
AB  - The chromo and SET domains are conserved sequence motifs present in chromosomal
      proteins that function in epigenetic control of gene expression, presumably by
      modulating higher order chromatin. Based on sequence information from the SET
      domain, we have isolated human (SUV39H1) and mouse (Suv39h1) homologues of the
      dominant Drosophila modifier of position-effect-variegation (PEV) Su(var)3-9.
      Mammalian homologues contain, in addition to the SET domain, the characteristic
      chromo domain, a combination that is also preserved in the Schizosaccharyomyces
      pombe silencing factor clr4. Chromatin-dependent gene regulation is demonstrated 
      by the potential of human SUV39H1 to increase repression of the pericentromeric
      white marker gene in transgenic flies. Immunodetection of endogenous
      Suv39h1/SUV39H1 proteins in a variety of mammalian cell lines reveals enriched
      distribution at heterochromatic foci during interphase and centromere-specific
      localization during metaphase. In addition, Suv39h1/SUV39H1 proteins associate
      with M31, currently the only other characterized mammalian SU(VAR) homologue.
      These data indicate the existence of a mammalian SU(VAR) complex and define
      Suv39h1/SUV39H1 as novel components of mammalian higher order chromatin.
FAU - Aagaard, L
AU  - Aagaard L
AD  - Research Institute of Molecular Pathology (IMP), The Vienna Biocenter, Dr.
      Bohrgasse 7, A-1030 Vienna, Austria.
FAU - Laible, G
AU  - Laible G
FAU - Selenko, P
AU  - Selenko P
FAU - Schmid, M
AU  - Schmid M
FAU - Dorn, R
AU  - Dorn R
FAU - Schotta, G
AU  - Schotta G
FAU - Kuhfittig, S
AU  - Kuhfittig S
FAU - Wolf, A
AU  - Wolf A
FAU - Lebersorger, A
AU  - Lebersorger A
FAU - Singh, P B
AU  - Singh PB
FAU - Reuter, G
AU  - Reuter G
FAU - Jenuwein, T
AU  - Jenuwein T
LA  - eng
SI  - GENBANK/AF019968
SI  - GENBANK/AF019969
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Cbx1 protein, mouse)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA Primers)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Heterochromatin)
RN  - 0 (Insect Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Su(var)3-9 protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Base Sequence
MH  - Centromere/metabolism
MH  - Chromosomal Proteins, Non-Histone/*genetics/isolation & purification/metabolism
MH  - Conserved Sequence
MH  - DNA Primers/genetics
MH  - Drosophila/genetics
MH  - *Drosophila Proteins
MH  - Fungal Proteins/genetics
MH  - Genes, Insect
MH  - Heterochromatin/genetics/metabolism
MH  - Humans
MH  - Insect Proteins/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Repressor Proteins/*genetics/isolation & purification/metabolism
MH  - Schizosaccharomyces/genetics
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
PMC - PMC1171278
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1093/emboj/18.7.1923 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 1;18(7):1923-38. doi: 10.1093/emboj/18.7.1923.