PMID- 10202152
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Apr 1
TI  - Phosphorylation by G1-specific cdk-cyclin complexes activates the nucleolar
      transcription factor UBF.
PG  - 1891-9
AB  - Transcription of rRNA genes by RNA polymerase I increases following serum
      stimulation of quiescent NIH 3T3 fibroblasts. To elucidate the mechanism
      underlying transcriptional activation during progression through the G1 phase of 
      the cell cycle, we have analyzed the activity and phosphorylation pattern of the 
      nucleolar transcription factor upstream binding factor (UBF). Using a combination
      of tryptic phosphopeptide mapping and site-directed mutagenesis, we have
      identified Ser484 as a direct target for cyclin-dependent kinase 4 (cdk4)-cyclin 
      D1- and cdk2-cyclin E-directed phosphorylation. Mutation of Ser484 impairs rDNA
      transcription in vivo and in vitro. The data demonstrate that UBF is regulated in
      a cell cycle-dependent manner and suggest a link between G1 cdks-cyclins, UBF
      phosphorylation and rDNA transcription activation.
FAU - Voit, R
AU  - Voit R
AD  - Division of Molecular Biology of the Cell II, German Cancer Research Center,
      D-69120 Heidelberg, Germany. R.Voit@DKFZ-Heidelberg.de
FAU - Hoffmann, M
AU  - Hoffmann M
FAU - Grummt, I
AU  - Grummt I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Cyclin E)
RN  - 0 (Cyclins)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Pol1 Transcription Initiation Complex Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA Precursors)
RN  - 0 (Transcription Factors)
RN  - 0 (transcription factor UBF)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (Cdk2 protein, mouse)
RN  - EC 2.7.11.22 (Cdk4 protein, mouse)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Binding Sites
MH  - *CDC2-CDC28 Kinases
MH  - Cell Nucleolus/metabolism
MH  - Cyclin D1/metabolism
MH  - Cyclin E/metabolism
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinase 4
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - Cyclins/*metabolism
MH  - DNA, Ribosomal/genetics
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - G1 Phase/genetics/*physiology
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - *Pol1 Transcription Initiation Complex Proteins
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - *Proto-Oncogene Proteins
MH  - RNA Precursors/biosynthesis
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcriptional Activation
PMC - PMC1171274
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1093/emboj/18.7.1891 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 1;18(7):1891-9. doi: 10.1093/emboj/18.7.1891.