PMID- 10202149
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Apr 1
TI  - Cloning and characterization of a cDNA encoding an A-kinase anchoring protein
      located in the centrosome, AKAP450.
PG  - 1858-68
AB  - A combination of protein kinase A type II (RII) overlay screening, database
      searches and PCR was used to identify a centrosomal A-kinase anchoring protein. A
      cDNA with an 11.7 kb open reading frame was characterized and found to correspond
      to 50 exons of genomic sequence on human chromosome 7q21-22. This cDNA clone
      encoded a 3908 amino acid protein of 453 kDa, that was designated AKAP450
      (DDBJ/EMBL/GenBank accession No. AJ131693). Sequence comparison demonstrated that
      the open reading frame contained a previously characterized cDNA encoding Yotiao,
      as well as the human homologue of AKAP120. Numerous coiled-coil structures were
      predicted from AKAP450, and weak homology to pericentrin, giantin and other
      structural proteins was observed. A putative RII-binding site was identified
      involving amino acid 2556 of AKAP450 by mutation analysis combined with RII
      overlay and an amphipatic helix was predicted in this region. Immunoprecipitation
      of RII from RIPA-buffer extracts of HeLa cells demonstrated co-precipitation of
      AKAP450. By immunofluorecent labeling with specific antibodies it was
      demonstrated that AKAP450 localized to centrosomes. Furthermore, AKAP450 was
      shown to co-purify in centrosomal preparations. The observation of two mRNAs and 
      several splice products suggests additional functions for the AKAP450 gene.
FAU - Witczak, O
AU  - Witczak O
AD  - Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo, PO
      Box 1112, Blindern N-0317 Oslo, Norway.
FAU - Skalhegg, B S
AU  - Skalhegg BS
FAU - Keryer, G
AU  - Keryer G
FAU - Bornens, M
AU  - Bornens M
FAU - Tasken, K
AU  - Tasken K
FAU - Jahnsen, T
AU  - Jahnsen T
FAU - Orstavik, S
AU  - Orstavik S
LA  - eng
SI  - GENBANK/AJ131693
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (A Kinase Anchor Proteins)
RN  - 0 (AKAP9 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Type II)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - A Kinase Anchor Proteins
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - *Carrier Proteins
MH  - Cell Line
MH  - Centrosome/*metabolism
MH  - Cloning, Molecular
MH  - Cyclic AMP-Dependent Protein Kinase Type II
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - *Cytoskeletal Proteins
MH  - DNA Primers/genetics
MH  - DNA, Complementary/*genetics
MH  - Exons
MH  - Female
MH  - Humans
MH  - Introns
MH  - Microtubule-Associated Proteins/*genetics/isolation & purification/*metabolism
MH  - Molecular Sequence Data
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Subcellular Fractions/metabolism
MH  - Tissue Distribution
PMC - PMC1171271
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1093/emboj/18.7.1858 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 1;18(7):1858-68. doi: 10.1093/emboj/18.7.1858.