PMID- 10202145
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Apr 1
TI  - DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel
      site, Ser20, requires tetramerization.
PG  - 1815-23
AB  - Upon DNA damage, p53 has been shown to be modified at a number of N-terminal
      phosphorylation sites including Ser15 and -33. Here we show that phosphorylation 
      is induced as well at a novel site, Ser20. Phosphorylation at Ser15, -20 and -33 
      can occur within minutes of DNA damage. Interestingly, while the DNA-binding
      activities of p53 appear to be dispensable, efficient phosphorylation at these
      three sites requires the tetramerization domain of p53. Substitution of an
      artificial tetramerization domain for this region also permits phosphorylation at
      the N-terminus, suggesting that oligomerization is important for DNA
      damage-induced signalling to p53.
FAU - Shieh, S Y
AU  - Shieh SY
AD  - Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
FAU - Taya, Y
AU  - Taya Y
FAU - Prives, C
AU  - Prives C
LA  - eng
GR  - CA58316/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 452VLY9402 (Serine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - *DNA Damage
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Conformation
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Serine/chemistry/metabolism
MH  - Signal Transduction
MH  - Tumor Suppressor Protein p53/*chemistry/genetics/*metabolism
PMC - PMC1171267
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1093/emboj/18.7.1815 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 1;18(7):1815-23. doi: 10.1093/emboj/18.7.1815.