PMID- 10202141
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Apr 1
TI  - Characterization of GAPCenA, a GTPase activating protein for Rab6, part of which 
      associates with the centrosome.
PG  - 1772-82
AB  - The Rab6 GTPase regulates intracellular transport at the level of the Golgi
      apparatus, probably in a retrograde direction. Here, we report the identification
      and characterization of a novel human Rab6-interacting protein named human
      GAPCenA (for 'GAP and centrosome-associated'). Primary sequence analysis
      indicates that GAPCenA displays similarities, within a central 200 amino acids
      domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the
      spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and
      Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP,
      specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2
      proteins. Immunofluorescence and cell fractionation experiments showed that
      GAPCenA is mainly cytosolic but that a minor pool is associated with the
      centrosome. Moreover, GAPCenA was found to form complexes with cytosolic
      gamma-tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA
      may be involved in the coordination of microtubule and Golgi dynamics during the 
      cell cycle.
FAU - Cuif, M H
AU  - Cuif MH
AD  - UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.
FAU - Possmayer, F
AU  - Possmayer F
FAU - Zander, H
AU  - Zander H
FAU - Bordes, N
AU  - Bordes N
FAU - Jollivet, F
AU  - Jollivet F
FAU - Couedel-Courteille, A
AU  - Couedel-Courteille A
FAU - Janoueix-Lerosey, I
AU  - Janoueix-Lerosey I
FAU - Langsley, G
AU  - Langsley G
FAU - Bornens, M
AU  - Bornens M
FAU - Goud, B
AU  - Goud B
LA  - eng
SI  - GENBANK/AJ011679
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Fungal Proteins)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Proteins)
RN  - 0 (RABGAP1 protein, human)
RN  - 0 (Rab6 protein)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tubulin)
RN  - 0 (ras GTPase-Activating Proteins)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*metabolism
MH  - Centrosome/*metabolism
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - Enzyme Activation
MH  - Fungal Proteins/genetics
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTPase-Activating Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Microtubule-Associated Proteins/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Proteins/*genetics/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Schizosaccharomyces/genetics
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/metabolism
MH  - Tubulin/metabolism
MH  - *rab GTP-Binding Proteins
MH  - ras GTPase-Activating Proteins
MH  - ras Proteins/*metabolism
PMC - PMC1171263
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1093/emboj/18.7.1772 [doi]
PST - ppublish
SO  - EMBO J. 1999 Apr 1;18(7):1772-82. doi: 10.1093/emboj/18.7.1772.