PMID- 10201973
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 8
DP  - 1999 Apr 15
TI  - Cutting edge: effects of an allergy-associated mutation in the human IL-4R alpha 
      (Q576R) on human IL-4-induced signal transduction.
PG  - 4385-9
AB  - A mutation in the human (hu) IL-4R alpha, Q576R, has been linked with allergy in 
      humans. Increased sensitivity of patients cells with this mutation to IL-4
      suggest that a Q576R change enhances IL-4 signaling. To directly test this
      hypothesis, we analyzed the ability of huIL-4R alpha cDNA bearing the Q576R and
      Y575F mutations to signal tyrosine phosphorylation, DNA-binding activity,
      proliferation, protection from apoptosis, and CD23 induction in response to
      huIL-4 in murine cells. Responses generated by the Q576R and Y575F mutants were
      similar to those of the wild-type receptor, using various concentrations of
      huIL-4 and times of stimulation. These results indicate that neither the Q576R
      nor the Y575F mutations have a significant direct effect on IL-4 signal
      transduction, and that hypersensitive induction of CD23 in cells derived from
      human allergy patients may be due to different and/or additional alterations in
      the IL-4 signaling pathway.
FAU - Wang, H Y
AU  - Wang HY
AD  - Department of Immunology, Jerome Holland Laboratories, American Red Cross,
      Rockville, MD 20855, USA.
FAU - Shelburne, C P
AU  - Shelburne CP
FAU - Zamorano, J
AU  - Zamorano J
FAU - Kelly, A E
AU  - Kelly AE
FAU - Ryan, J J
AU  - Ryan JJ
FAU - Keegan, A D
AU  - Keegan AD
LA  - eng
GR  - AI38985/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IRS1 protein, human)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Interleukin-4)
RN  - 0 (STAT6 Transcription Factor)
RN  - 0 (STAT6 protein, human)
RN  - 0 (Stat6 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0RH81L854J (Glutamine)
RN  - 42HK56048U (Tyrosine)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Amino Acid Substitution/genetics/immunology
MH  - Animals
MH  - Apoptosis/genetics/immunology
MH  - Arginine/genetics
MH  - Cell Line
MH  - DNA-Binding Proteins/biosynthesis
MH  - Glutamine/genetics
MH  - Humans
MH  - Hypersensitivity/*genetics/immunology
MH  - Insulin Receptor Substrate Proteins
MH  - Lymphocyte Activation/genetics
MH  - Mice
MH  - Mutagenesis, Site-Directed/*immunology
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Receptor, Insulin/metabolism
MH  - Receptors, IgE/biosynthesis
MH  - Receptors, Interleukin-4/*genetics/physiology
MH  - STAT6 Transcription Factor
MH  - Signal Transduction/genetics/*immunology
MH  - Trans-Activators/metabolism
MH  - Tyrosine/genetics/metabolism
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:01
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:01 [medline]
PHST- 1999/04/14 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Apr 15;162(8):4385-9.