PMID- 10201933
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20101118
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 7
DP  - 1999 Apr 1
TI  - Molecular cloning of a glycosylphosphatidylinositol-anchored molecule CDw108.
PG  - 4094-100
AB  - CDw108, also known as the John-Milton-Hagen human blood group Ag, is an 80-kDa
      glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is
      preferentially expressed on activated lymphocytes and E. The molecular
      characteristics and biological function of the CDw108 were not clarified
      previously. In this manuscript, we identify the cDNA clone containing the entire 
      coding sequence of the CDw108 gene and report its molecular characteristics. The 
      1998-base pairs of the open reading frame of the cloned cDNA encoded a protein of
      666 amino acids (aa), including the 46 aa of the signal peptide and the 19 aa of 
      the GPI-anchor motif. Thus, the membrane-anchoring form of CDw108 was the 602 aa,
      and the estimated molecular mass of the unglycosylated form was 68 kDa. The RGD
      (Arg-Gly-Asp) cell attachment sequence and the five potential N-linked
      glycosylation sites were located on the membrane-anchoring form. Flow cytometric 
      and immunoprecipitation analyses of the CDw108 cDNA transfectants confirmed that 
      the cloned cDNA encoded the native form of CDw108. The CDw108 mRNA was expressed 
      in activated PBMCs as well as in the spleen, thymus, testis, placenta, and brain,
      but was not expressed in any other tissues tested. Radiation hybrid mapping
      indicated that the CDw108 gene was located in the middle of the long arm of
      chromosome 15 (15q23-24). This molecular information will be critical for
      understanding the biological function of the CDw108 Ag.
FAU - Yamada, A
AU  - Yamada A
AD  - Cancer Vaccine Development Division, Kurume University Research Center for
      Innovative Cancer Therapy, Department of Immunology, Japan.
      akiymd@med.kurume-u.ac.jp
FAU - Kubo, K
AU  - Kubo K
FAU - Takeshita, T
AU  - Takeshita T
FAU - Harashima, N
AU  - Harashima N
FAU - Kawano, K
AU  - Kawano K
FAU - Mine, T
AU  - Mine T
FAU - Sagawa, K
AU  - Sagawa K
FAU - Sugamura, K
AU  - Sugamura K
FAU - Itoh, K
AU  - Itoh K
LA  - eng
SI  - GENBANK/AF069493
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (Blood Group Antigens)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (SEMA7A protein, human)
RN  - 0 (Semaphorins)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD/chemistry/*genetics/metabolism
MH  - Base Sequence
MH  - Blood Group Antigens/*genetics/immunology
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 15/immunology
MH  - Cloning, Molecular
MH  - GPI-Linked Proteins
MH  - Glycosylphosphatidylinositols/*metabolism
MH  - Humans
MH  - Membrane Glycoproteins/chemistry/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics/immunology
MH  - *Semaphorins
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:01
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:01 [medline]
PHST- 1999/04/14 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Apr 1;162(7):4094-100.