PMID- 10201920
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 7
DP  - 1999 Apr 1
TI  - Triggering of effector functions on a CD8+ T cell clone upon the aggregation of
      an activatory CD94/kp39 heterodimer.
PG  - 3996-4002
AB  - Some T lymphocytes express the CD94 Ag, which is known to form heterodimers with 
      members of the NKG2 family. We have studied the expression pattern and function
      of CD94 heterodimers in different alphabeta or gammadelta T cell clones. Most of 
      the CD94+NKG2A- T cells have a low to intermediate expression of CD94 Ag. The
      cross-linking of the CD94/NKG2 heterodimer in one of these CD8 alphabeta
      CD94+NKG2A- T cell clones (K14B06) was able to: 1) increase the intracellular
      concentration of Ca2+, 2) induce the up-regulation of CD25 Ag expression and the 
      secretion of IFN-gamma, and 3) trigger redirected cytotoxicity in a
      TCR-independent manner. This activatory property was not shared by any other
      costimulatory molecule expressed by the K14B06 T cell clone, including CD8, CD28,
      CD45, CD69, or CD2 Ags. The immunoprecipitation of CD94 heterodimer showed a
      39-kDa band with a similar m.w. to the activatory heterodimer found on some NK
      clones. A novel form of the NKG2 family (NKG2H) was identified in K14B06. NKG2H
      protein represents an alternative spliced form of the NKG2E gene, displaying a
      charged residue in the transmembrane portion and a cytoplasmic tail that lacks
      immunoreceptor tyrosine-based inhibitory motifs. The expression of NKG2H in the
      cell membrane through its association to CD94 and DAP-12 molecules supports that 
      it could form part of the activatory CD94/Kp39 heterodimer present on K14B06
      cells.
FAU - Bellon, T
AU  - Bellon T
AD  - Seccion de Inmunologia, Hospital de la Princesa, Madrid, Spain.
FAU - Heredia, A B
AU  - Heredia AB
FAU - Llano, M
AU  - Llano M
FAU - Minguela, A
AU  - Minguela A
FAU - Rodriguez, A
AU  - Rodriguez A
FAU - Lopez-Botet, M
AU  - Lopez-Botet M
FAU - Aparicio, P
AU  - Aparicio P
LA  - eng
SI  - GENBANK/AF078550
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (CD3 Complex)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRC3 protein, human)
RN  - 0 (KLRD1 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Receptors, Natural Killer Cell)
RN  - 82115-62-6 (Interferon-gamma)
RN  - SY7Q814VUP (Calcium)
SB  - AIM
SB  - IM
SB  - X
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/immunology/*metabolism
MH  - CD3 Complex/immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Calcium/metabolism
MH  - Cell Line, Transformed
MH  - Clone Cells
MH  - Cytotoxicity, Immunologic
MH  - Dimerization
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - *Lectins, C-Type
MH  - *Lymphocyte Activation
MH  - Membrane Glycoproteins/immunology/*metabolism
MH  - Molecular Sequence Data
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily D
MH  - Receptor Aggregation/*immunology
MH  - Receptors, Immunologic/genetics/immunology/isolation & purification/*metabolism
MH  - Receptors, Interleukin-2/metabolism
MH  - Receptors, Natural Killer Cell
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:01
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:01 [medline]
PHST- 1999/04/14 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Apr 1;162(7):3996-4002.