PMID- 10201374
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20181201
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 398
IP  - 6726
DP  - 1999 Apr 1
TI  - A new secreted protein that binds to Wnt proteins and inhibits their activities.
PG  - 431-6
AB  - The Wnt proteins constitute a large family of extracellular signalling molecules 
      that are found throughout the animal kingdom and are important for a wide variety
      of normal and pathological developmental processes. Here we describe
      Wnt-inhibitory factor-1 (WIF-1), a secreted protein that binds to Wnt proteins
      and inhibits their activities. WIF-1 is present in fish, amphibia and mammals,
      and is expressed during Xenopus and zebrafish development in a complex pattern
      that includes paraxial presomitic mesoderm, notochord, branchial arches and
      neural crest derivatives. We use Xenopus embryos to show that WIF-1
      overexpression affects somitogenesis (the generation of trunk mesoderm segments),
      in agreement with its normal expression in paraxial mesoderm. In vitro, WIF-1
      binds to Drosophila Wingless and Xenopus Wnt8 produced by Drosophila S2 cells.
      Together with earlier results obtained with the secreted Frizzled-related
      proteins, our results indicate that Wnt proteins interact with structurally
      diverse extracellular inhibitors, presumably to fine-tune the spatial and
      temporal patterns of Wnt activity.
FAU - Hsieh, J C
AU  - Hsieh JC
AD  - Department of Molecular Biology and Genetics, Howard Hughes Medical Institute,
      Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
FAU - Kodjabachian, L
AU  - Kodjabachian L
FAU - Rebbert, M L
AU  - Rebbert ML
FAU - Rattner, A
AU  - Rattner A
FAU - Smallwood, P M
AU  - Smallwood PM
FAU - Samos, C H
AU  - Samos CH
FAU - Nusse, R
AU  - Nusse R
FAU - Dawid, I B
AU  - Dawid IB
FAU - Nathans, J
AU  - Nathans J
LA  - eng
SI  - GENBANK/AF122922
SI  - GENBANK/AF122923
SI  - GENBANK/AF122924
SI  - GENBANK/AF122925
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (WIF1 protein, human)
RN  - 0 (Wif1 protein, mouse)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt1 Protein)
RN  - 0 (Xenopus Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (wg protein, Drosophila)
RN  - 0 (wnt8a protein, Xenopus)
RN  - 0 (wnt8b protein, zebrafish)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Carrier Proteins/genetics/isolation & purification/*metabolism
MH  - Cricetinae
MH  - *Drosophila Proteins
MH  - Extracellular Matrix Proteins
MH  - Humans
MH  - Immunoglobulin G/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/antagonists & inhibitors/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - *Repressor Proteins
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Wnt Proteins
MH  - Wnt1 Protein
MH  - Xenopus
MH  - Xenopus Proteins
MH  - Zebrafish
MH  - *Zebrafish Proteins
EDAT- 1999/04/14 02:03
MHDA- 2001/03/23 10:01
CRDT- 1999/04/14 02:03
PHST- 1999/04/14 02:03 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/04/14 02:03 [entrez]
AID - 10.1038/18899 [doi]
PST - ppublish
SO  - Nature. 1999 Apr 1;398(6726):431-6. doi: 10.1038/18899.