PMID- 10200558
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20190628
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 6
IP  - 2
DP  - 1999 Feb
TI  - Alix, a novel mouse protein undergoing calcium-dependent interaction with the
      apoptosis-linked-gene 2 (ALG-2) protein.
PG  - 124-9
AB  - ALG-2 is a EF hand calcium binding protein with sequence homologies to
      calmodulin. Vito et al have shown that ALG-2 expression is required for apoptosis
      following a number of death stimuli,1 although nothing is known about the
      effectors which underlie ALG-2 function. Here we have used ALG-2 as bait in a
      yeast two hybrid screen of a mouse brain cDNA library. We found that ALG-2 binds 
      to itself and to a novel protein that we call ALG-2 interacting protein X, Alix. 
      Using co-immunoprecipitation experiments, we confirmed ALG-2/ALG-2 binding and
      demonstrated that this interaction is calcium independent. ALG-2/Alix interaction
      was also validated by co-immunoprecipitation, but in this case, the binding was
      found to be strictly calcium dependent. Alix seems highly conserved throughout
      evolution since it shows significant homologies to a putative C. elegans protein 
      (YNK-1) and to proteins of A. nidulans (PalA) and S. cerevisiae (BRO1). Alix is a
      potential regulator or downstream effector of ALG-2 action.
FAU - Missotten, M
AU  - Missotten M
AD  - Serono Pharmaceutical Research Institute, 14 Chemin des Aulx, 1228 Plan les
      Ouates, Geneva, Switzerland.
FAU - Nichols, A
AU  - Nichols A
FAU - Rieger, K
AU  - Rieger K
FAU - Sadoul, R
AU  - Sadoul R
LA  - eng
SI  - GENBANK/AJ005073
SI  - GENBANK/AJ005074
PT  - Journal Article
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (ALX-1 protein, C elegans)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (PALA protein, Emericella nidulans)
RN  - 0 (Pdcd6 protein, mouse)
RN  - 0 (Pdcd6ip protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Apoptosis Regulatory Proteins
MH  - Brain/metabolism
MH  - *Caenorhabditis elegans Proteins
MH  - Calcium/pharmacology
MH  - Calcium-Binding Proteins/*genetics/metabolism
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - Evolution, Molecular
MH  - Fungal Proteins/genetics
MH  - Gene Expression Regulation/genetics
MH  - Homeodomain Proteins/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protein Binding
MH  - RNA, Messenger/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Transfection
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:01
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:01 [medline]
PHST- 1999/04/14 00:00 [entrez]
AID - 10.1038/sj.cdd.4400456 [doi]
PST - ppublish
SO  - Cell Death Differ. 1999 Feb;6(2):124-9. doi: 10.1038/sj.cdd.4400456.