PMID- 10200264
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 8
DP  - 1999 Apr 13
TI  - Universal conservation in translation initiation revealed by human and archaeal
      homologs of bacterial translation initiation factor IF2.
PG  - 4342-7
AB  - Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes by
      initiation factor (IF) IF2 and in eukaryotes by eIF2. The discovery of both IF2
      and eIF2 homologs in yeast and archaea suggested that these microbes possess an
      evolutionarily intermediate protein synthesis apparatus. We describe the
      identification of a human IF2 homolog, and we demonstrate by using in vivo and in
      vitro assays that human IF2 functions as a translation factor. In addition, we
      show that archaea IF2 can substitute for its yeast homolog both in vivo and in
      vitro. We propose a universally conserved function for IF2 in facilitating the
      proper binding of initiator methionyl-tRNA to the ribosomal P site.
FAU - Lee, J H
AU  - Lee JH
AD  - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and 
      Human Development, National Institutes of Health, Bethesda, MD 20892-2716, USA.
FAU - Choi, S K
AU  - Choi SK
FAU - Roll-Mecak, A
AU  - Roll-Mecak A
FAU - Burley, S K
AU  - Burley SK
FAU - Dever, T E
AU  - Dever TE
LA  - eng
SI  - GENBANK/AF078035
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Archaeal Proteins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (IF2 protein, archaeal)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Prokaryotic Initiation Factor-2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Archaea/*genetics
MH  - Archaeal Proteins/chemistry/genetics
MH  - Bacterial Proteins/chemistry/genetics
MH  - Conserved Sequence
MH  - Eukaryotic Initiation Factor-2/*chemistry/*genetics
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - *Peptide Initiation Factors
MH  - Phylogeny
MH  - Prokaryotic Initiation Factor-2
MH  - Protein Biosynthesis
MH  - Saccharomyces cerevisiae/genetics
PMC - PMC16334
EDAT- 1999/04/14 02:02
MHDA- 2000/05/20 09:00
CRDT- 1999/04/14 02:02
PHST- 1999/04/14 02:02 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 1999/04/14 02:02 [entrez]
AID - 10.1073/pnas.96.8.4342 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4342-7. doi: 10.1073/pnas.96.8.4342.