PMID- 10200233
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 81
IP  - 4
DP  - 1999 Apr
TI  - Cloning and expression of the human kv4.3 potassium channel.
PG  - 1974-7
AB  - We report on the cloning and expression of hKv4.3, a fast inactivating,
      transient, A-type potassium channel found in both heart and brain that is 91%
      homologous to the rat Kv4.3 channel. Two isoforms of hKv4.3 were cloned. One is
      full length (hKv4.3 long), and the other has a 19 amino acid deletion (hKv4.3
      short). RT-PCR shows that the brain contains both forms of the channel RNA,
      whereas the heart predominantly has the longer version. Both versions of the
      channel were expressed in Xenopus oocytes, and both contain a significant window 
      or noninactivating current seen near potentials of -30 to -40 mV. The
      inactivation curve for hKv4.3 short is shifted 10 mV positive relative to hKv4.3 
      long. This causes the peak window current for the short version to occur near -30
      mV and the peak for the longer version to be at -40 mV. There was little
      difference in the recovery from inactivation or in the kinetics of inactivation
      between the two isoforms of the channel.
FAU - Dilks, D
AU  - Dilks D
AD  - CNS Research, Wyeth-Ayerst Research, Princeton, New Jersey 08543, USA.
FAU - Ling, H P
AU  - Ling HP
FAU - Cockett, M
AU  - Cockett M
FAU - Sokol, P
AU  - Sokol P
FAU - Numann, R
AU  - Numann R
LA  - eng
SI  - GENBANK/AF187963
SI  - GENBANK/AF187964
PT  - Journal Article
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (KCND3 protein, human)
RN  - 0 (Kcnd3 protein, rat)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Voltage-Gated)
RN  - 0 (Shal Potassium Channels)
SB  - IM
MH  - Animals
MH  - Brain Chemistry/physiology
MH  - Cloning, Molecular
MH  - Gene Expression/physiology
MH  - Humans
MH  - Kinetics
MH  - Membrane Potentials/physiology
MH  - Molecular Sequence Data
MH  - Muscle Fibers, Skeletal/*chemistry
MH  - Myocardium/chemistry/cytology
MH  - Neurons/*chemistry
MH  - Oocytes/physiology
MH  - Patch-Clamp Techniques
MH  - Potassium Channels/*genetics/*metabolism
MH  - *Potassium Channels, Voltage-Gated
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Shal Potassium Channels
MH  - Xenopus
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:01
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:01 [medline]
PHST- 1999/04/14 00:00 [entrez]
AID - 10.1152/jn.1999.81.4.1974 [doi]
PST - ppublish
SO  - J Neurophysiol. 1999 Apr;81(4):1974-7. doi: 10.1152/jn.1999.81.4.1974.