PMID- 10199918
OWN - NLM
STAT- MEDLINE
DCOM- 19990602
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 49
IP  - 5
DP  - 1999 May
TI  - Cloning and chromosomal mapping of a gene isolated from thymic stromal cells
      encoding a new mouse type II membrane serine protease, epithin, containing four
      LDL receptor modules and two CUB domains.
PG  - 420-8
AB  - We cloned and sequenced a mouse gene encoding a new type of membrane bound serine
      protease (epithin) containing a multidomain structure. The initial cDNA clone was
      found previously in a polymerase chain reaction (PCR)-based subtractive library
      generated from fetal thymic stromal cells, and the message was shown to be highly
      expressed in a thymic epithelial nurse cell line. A clone isolated from a severe 
      combined immunodeficiency (SCID) thymus library and extended to its full length
      at the 5' end with the RACE technique contains an open reading frame of 902 amino
      acids. Based on the sequence of this clone, the predicted protein structure is a 
      type II membrane protein with a C-terminal serine protease domain linked to the
      membrane by four low density lipoprotein receptor modules and two CUB domains.
      High message expression by northern blotting was detected in intestine, kidney,
      lung, SCID, and Rag-2(-/-) thymus, and 2-deoxyguanosine-treated fetal thymic
      rudiment, but not in skeletal muscle, liver, heart, testis, and brain. Sorted MHC
      class II+ and II- fetal thymic stromal cells were positive for expression by
      reverse transcriptase-PCR, whereas CD45(+) thymocytes were not. The gene was
      found in chicken and multiple mammalian species under low stringency Southern
      hybridization conditions. Under high stringency conditions, only a single gene
      per haploid genome was identified in the mouse. This gene, Prss14 (protease,
      serine, 14), was mapped to mouse chromosome 9 and is closely linked to the Fli1
      (Friend leukemia integration 1) gene.
FAU - Kim, M G
AU  - Kim MG
AD  - Laboratory of Cellular and Molecular Immunology, National Institute of Allergy
      and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0420, 
      USA.
FAU - Chen, C
AU  - Chen C
FAU - Lyu, M S
AU  - Lyu MS
FAU - Cho, E G
AU  - Cho EG
FAU - Park, D
AU  - Park D
FAU - Kozak, C
AU  - Kozak C
FAU - Schwartz, R H
AU  - Schwartz RH
LA  - eng
SI  - GENBANK/AF042822
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, LDL)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (matriptase)
RN  - EC 3.4.21.109 (St14 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Membrane Proteins
MH  - Mice
MH  - Mice, SCID
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Receptors, LDL/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/*genetics
MH  - Stromal Cells
MH  - Thymus Gland/cytology/*enzymology
MH  - Tissue Distribution
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - 10.1007/s002510050515 [doi]
PST - ppublish
SO  - Immunogenetics. 1999 May;49(5):420-8. doi: 10.1007/s002510050515.