PMID- 10199912
OWN - NLM
STAT- MEDLINE
DCOM- 19990602
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 49
IP  - 5
DP  - 1999 May
TI  - Molecular cloning of the human gene STK10 encoding lymphocyte-oriented kinase,
      and comparative chromosomal mapping of the human, mouse, and rat homologues.
PG  - 369-75
AB  - LOK is a new and unique member of the STE20 family with serine/threonine kinase
      activity, and its expression is restricted mostly to lymphoid cells in mice. We
      cloned the cDNA encoding the human homologue of LOK. The amino acid sequence
      deduced from the cDNA shows a high similarity to that of mouse LOK, with 88%
      identity as a whole. The kinase domains at the N-terminus and the coiled-coil
      regions at the C-terminus are particularly conserved, showing 98% and 93%
      identity, respectively. Western blot analysis with mouse LOK-specific antibody
      detected 130 000 Mr LOK proteins in human and rat lymphoid cell lines and
      tissues. The gene encoding the LOK (STK10/Stk10) gene was mapped by fluorescence 
      in situ hybridization to chromosome 5q35.1 in human, chromosome 11A4 in mouse,
      and chromosome 10q12.3 in rat. By virtue of polymorphic CA repeats found in the
      3' untranslated region of the mouse Stk10 gene, the Stk10 locus was further
      pinpointed to chromosome 11 between D11Mit53 and D11Mit84, using the
      intersubspecific backcross mapping panel. These results established STK10 as a
      new marker of human chromosome 5 to define the syntenic boundary of human
      chromosomes 5 and 16 on mouse chromosome 11.
FAU - Kuramochi, S
AU  - Kuramochi S
AD  - Department of Immunology, The Tokyo Metropolitan Institute of Medical Science,
      3-18-22, Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Okamoto, M
AU  - Okamoto M
FAU - Kitamura, F
AU  - Kitamura F
FAU - Yonekawa, H
AU  - Yonekawa H
FAU - Karasuyama, H
AU  - Karasuyama H
LA  - eng
SI  - GENBANK/AB015718
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Stk10 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary/genetics
MH  - Dinucleotide Repeats
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocytes
MH  - Lymphocytes/*enzymology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic
MH  - Protein-Serine-Threonine Kinases/*genetics
MH  - Rats
MH  - Species Specificity
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - 10.1007/s002510050509 [doi]
PST - ppublish
SO  - Immunogenetics. 1999 May;49(5):369-75. doi: 10.1007/s002510050509.