PMID- 10199401
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 1
DP  - 1999 Apr 2
TI  - NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA
      polymerase II elongation.
PG  - 41-51
AB  - DRB is a classic inhibitor of transcription elongation by RNA polymerase II (pol 
      II). Since DRB generally affects class II genes, factors involved in this process
      must play fundamental roles in pol II elongation. Recently, two elongation
      factors essential for DRB action were identified, namely DSIF and P-TEFb. Here we
      describe the identification and purification from HeLa nuclear extract of a third
      protein factor required for DRB-sensitive transcription. This factor, termed
      negative elongation factor (NELF), cooperates with DSIF and strongly represses
      pol II elongation. This repression is reversed by P-TEFb-dependent
      phosphorylation of the pol II C-terminal domain. NELF is composed of five
      polypeptides, the smallest of which is identical to RD, a putative RNA-binding
      protein of unknown function. This study reveals a molecular mechanism for DRB
      action and a regulatory network of positive and negative elongation factors.
FAU - Yamaguchi, Y
AU  - Yamaguchi Y
AD  - Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama,
      Japan.
FAU - Takagi, T
AU  - Takagi T
FAU - Wada, T
AU  - Wada T
FAU - Yano, K
AU  - Yano K
FAU - Furuya, A
AU  - Furuya A
FAU - Sugimoto, S
AU  - Sugimoto S
FAU - Hasegawa, J
AU  - Hasegawa J
FAU - Handa, H
AU  - Handa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SUPT4H1 protein, human)
RN  - 0 (SUPT5H protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (negative elongation factor)
RN  - 53-85-0 (Dichlororibofuranosylbenzimidazole)
RN  - EC 2.7.11.- (Positive Transcriptional Elongation Factor B)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - Dichlororibofuranosylbenzimidazole/pharmacology
MH  - Dimerization
MH  - HeLa Cells
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*physiology
MH  - Positive Transcriptional Elongation Factor B
MH  - Protein-Serine-Threonine Kinases/metabolism/physiology
MH  - RNA Polymerase II/*antagonists & inhibitors/genetics
MH  - RNA-Binding Proteins/physiology
MH  - Repressor Proteins/*physiology
MH  - Transcription Factors/genetics/isolation & purification/*physiology
MH  - Transcriptional Elongation Factors
MH  - Tumor Cells, Cultured
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0092-8674(00)80713-8 [pii]
AID - 10.1016/s0092-8674(00)80713-8 [doi]
PST - ppublish
SO  - Cell. 1999 Apr 2;97(1):41-51. doi: 10.1016/s0092-8674(00)80713-8.