PMID- 10198631
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20161124
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 3
DP  - 1999 Mar
TI  - The proapoptotic activity of the Bcl-2 family member Bim is regulated by
      interaction with the dynein motor complex.
PG  - 287-96
AB  - Bcl-2 family members that have only a single Bcl-2 homology domain, BH3, are
      potent inducers of apoptosis, and some appear to play a critical role in
      developmentally programmed cell death. We examined the regulation of the
      proapoptotic activity of the BH3-only protein Bim. In healthy cells, most Bim
      molecules were bound to LC8 cytoplasmic dynein light chain and thereby
      sequestered to the microtubule-associated dynein motor complex. Certain apoptotic
      stimuli disrupted the interaction between LC8 and the dynein motor complex. This 
      freed Bim to translocate together with LC8 to Bcl-2 and to neutralize its
      antiapoptotic activity. This process did not require caspase activity and
      therefore constitutes an initiating event in apoptosis signaling.
FAU - Puthalakath, H
AU  - Puthalakath H
AD  - Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital,
      Victoria, Australia.
FAU - Huang, D C
AU  - Huang DC
FAU - O'Reilly, L A
AU  - O'Reilly LA
FAU - King, S M
AU  - King SM
FAU - Strasser, A
AU  - Strasser A
LA  - eng
GR  - GM51293/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BCL2L11 protein, human)
RN  - 0 (Bcl-2-Like Protein 11)
RN  - 0 (Bcl2l11 protein, mouse)
RN  - 0 (Carrier Proteins)
RN  - 0 (Caspase Inhibitors)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Molecular Motor Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.6.4.2 (Dyneins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Apoptosis/drug effects/radiation effects
MH  - Apoptosis Regulatory Proteins
MH  - Bcl-2-Like Protein 11
MH  - Binding Sites
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Caspase Inhibitors
MH  - Caspases/metabolism
MH  - Cell Line
MH  - Dimerization
MH  - *Drosophila Proteins
MH  - Dyneins
MH  - Gene Library
MH  - Humans
MH  - *Membrane Proteins
MH  - Mice
MH  - Microtubules/metabolism
MH  - Molecular Motor Proteins/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Isoforms/genetics/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Saccharomyces cerevisiae/genetics
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S1097-2765(00)80456-6 [pii]
PST - ppublish
SO  - Mol Cell. 1999 Mar;3(3):287-96.