PMID- 10198427
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 9
DP  - 1999 May 1
TI  - Npw38, a novel nuclear protein possessing a WW domain capable of activating basal
      transcription.
PG  - 1957-65
AB  - We have found a novel cDNA encoding a 265 amino acid protein possessing a WW
      domain in our full-length cDNA bank. The WW domain was sandwiched between an
      acidic region and an acidic-basic amino acid repetitive region. In vitro
      transcription/translation of the cDNA produced a 38 kDa product that was also
      found in the cell lysate by western blot analysis. Thus this protein is named the
      nuclear protein containing a WW domain with a molecular mass of 38 kDa, Npw38.
      Immunofluorescence studies and expression of a fusion protein to a green
      fluorescent protein revealed that this protein is localized in the nucleus. Npw38
      was shown to be capable of binding to a poly(rG) resin. Interestingly, the WW
      domain of Npw38 was found to function as a transcriptional activator in CHO cells
      using the GAL4 DNA-binding fusion system. Furthermore, the WW domains of human
      YAP and Pin1 were demonstrated to have a similar transcription-promoting
      activity. Combined mutation of the conserved first and second Trp residues and a 
      hydrophobic triplet of TyrTyrTrp in the WW domain of Npw38 abolished the
      transcription-promoting activity, but single mutations of these sites did not.
      These results suggest that some WW domains potentially possess
      transcription-promoting activity in mammalian cells.
FAU - Komuro, A
AU  - Komuro A
AD  - Kato Cytoprotein Network Project, ERATO, Japan Science and Technology Corporation
      (JST), c/o Sagami Chemical Research Center, Nishi-Ohnuma 4-4-1, Sagamihara,
      Kanagawa 229-0012, Japan.
FAU - Saeki, M
AU  - Saeki M
FAU - Kato, S
AU  - Kato S
LA  - eng
SI  - GENBANK/AB016533
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PQBP1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - *Carrier Proteins
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Nuclear Proteins/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Trans-Activators/genetics/*metabolism
PMC - PMC148407
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - gkc336 [pii]
AID - 10.1093/nar/27.9.1957 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 May 1;27(9):1957-65. doi: 10.1093/nar/27.9.1957.