PMID- 10198256
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20211203
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 2
DP  - 1999 Apr 13
TI  - Phosphorylation of human MAD1 by the BUB1 kinase in vitro.
PG  - 589-95
AB  - The BUB/MAD signaling pathway monitors attachment of chromosomes to spindle poles
      in mitotic cells. Mutations of the human BUB1 locus were identified in cancer
      cells exhibiting an unstable chromosomal complement. We report that the human
      BUB3 gene maps to a site on chromosome 10 subject to frequent modification in
      cancers. Thus, defects in BUB/MAD signaling may contribute to genetic instability
      and to cancer progression. In vitro, BUB1 and BUB3 proteins form a complex of
      monomers of each protein. These proteins interact with the human MAD1 gene
      product, a target of the HTLV-1 tax oncogene. This multiprotein complex exhibits 
      a kinase activity with a requirement for lysine 821 in the BUB1 kinase motif,
      resulting in BUB1 autophosphorylation and phosphorylation of associated MAD1.
CI  - Copyright 1999 Academic Press.
FAU - Seeley, T W
AU  - Seeley TW
AD  - Chiron Corporation, 4560 Horton Street, Emeryville, California, 94608, USA.
FAU - Wang, L
AU  - Wang L
FAU - Zhen, J Y
AU  - Zhen JY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (BUB3 protein, human)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Fungal Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MAD1L1 protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Poly-ADP-Ribose Binding Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (RAE1 protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (protein kinase modulator)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (BUB1 protein, human)
RN  - EC 2.7.11.1 (Bub1 spindle checkpoint protein)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adenosine Triphosphate/metabolism
MH  - Amino Acid Substitution
MH  - Calcium-Binding Proteins/genetics/metabolism
MH  - Carrier Proteins/pharmacology
MH  - *Cell Cycle Proteins
MH  - Fungal Proteins/genetics/metabolism
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Lysine/genetics/metabolism
MH  - Mutation
MH  - *Nuclear Matrix-Associated Proteins
MH  - Nuclear Proteins/genetics/*metabolism
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Phosphoproteins/genetics/*metabolism
MH  - Phosphorylation/drug effects
MH  - Poly-ADP-Ribose Binding Proteins
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Biosynthesis
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/chemistry/genetics/*metabolism
MH  - Protein Serine-Threonine Kinases
MH  - Proteins/genetics/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Repressor Proteins
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0006-291X(99)90514-4 [pii]
AID - 10.1006/bbrc.1999.0514 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 13;257(2):589-95. doi:
      10.1006/bbrc.1999.0514.