PMID- 10198225
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 2
DP  - 1999 Apr 13
TI  - Physical interaction between interleukin-12 receptor beta 2 subunit and Jak2
      tyrosine kinase: Jak2 associates with cytoplasmic membrane-proximal region of
      interleukin-12 receptor beta 2 via amino-terminus.
PG  - 400-4
AB  - IL-12 is a heterodimeric cytokine, composed of p40 and p35 subunits, that exerts 
      its biological effects by binding to specific cell surface receptors. Two human
      IL-12 receptor proteins, designated IL-12R beta 1 and IL-12R beta 2, have been
      previously identified. IL-12R beta 2 has box 1 motif, box 2 motif, and three
      tyrosine residues in its cytoplasmic domain. In response to IL-12, Jak2 and Tyk2,
      family members of Janus family protein tyrosine kinases, are phosphorylated in
      PHA-activated T lymphocytes. The present study demonstrates that Jak2 binds to
      the cytoplasmic membrane-proximal region of IL-12R beta 2, and box 2 motif and
      tyrosine residues in the cytoplasmic domain were not required for binding. The
      amino-terminus of Jak2 is necessary for association with IL-12R beta 2.
CI  - Copyright 1999 Academic Press.
FAU - Yamamoto, K
AU  - Yamamoto K
AD  - First Department of Internal Medicine, Tokyo Medical and Dental University,
      Tokyo, Japan. kohyamamoto.med1@med.tmd.ac.jp
FAU - Shibata, F
AU  - Shibata F
FAU - Miura, O
AU  - Miura O
FAU - Kamiyama, R
AU  - Kamiyama R
FAU - Hirosawa, S
AU  - Hirosawa S
FAU - Miyasaka, N
AU  - Miyasaka N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-12)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, EphB4)
RN  - EC 2.7.10.1 (Receptors, Eph Family)
RN  - EC 2.7.10.2 (JAK2 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - COS Cells
MH  - Cell Membrane/*metabolism
MH  - Cytoplasm/metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Janus Kinase 2
MH  - Phosphorylation
MH  - Phosphotyrosine/metabolism
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein-Tyrosine Kinases/chemistry/genetics/*metabolism
MH  - Proteins/genetics/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Receptor Protein-Tyrosine Kinases/genetics/metabolism
MH  - Receptor, EphB4
MH  - Receptors, Eph Family
MH  - Receptors, Interleukin/chemistry/genetics/*metabolism
MH  - Receptors, Interleukin-12
MH  - Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism
MH  - Sequence Deletion
MH  - Transfection
MH  - Tyrosine/genetics/metabolism
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0006-291X(99)90479-5 [pii]
AID - 10.1006/bbrc.1999.0479 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 13;257(2):400-4. doi:
      10.1006/bbrc.1999.0479.