PMID- 10198163
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20071115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 57
IP  - 2
DP  - 1999 Apr 15
TI  - Characterization and expression pattern of the frizzled gene Fzd9, the mouse
      homolog of FZD9 which is deleted in Williams-Beuren syndrome.
PG  - 235-48
AB  - The frizzled gene family is conserved from insects to mammals and codes for
      putative Wnt receptors that share a cysteine-rich extracellular domain and seven 
      transmembrane domains. We previously identified a novel frizzled gene, FZD3, now 
      renamed FZD9, in the Williams-Beuren syndrome (WBS) deletion region at
      chromosomal band 7q11.23 and showed that its product can interact with the
      Drosophila wingless protein. Here, we report the characterization of the mouse
      homolog Fzd9. The Fzd9 gene produces a 2.4-kb transcript encoding a
      592-amino-acid protein with 95% identity to the human FZD9. Fzd9 was mapped to
      the conserved syntenic region on distal mouse chromosome 5. By RNA in situ
      hybridization studies of whole-mount embryos and sections we delineated the
      temporal and spatial expression patterns in the neural tube, trunk skeletal
      muscle precursors (myotomes), limb skeletal anlagen, craniofacial regions, and
      nephric ducts. In adult mouse tissue, the Fzd9 transcript is abundantly present
      in heart, brain, testis, and skeletal muscle. In testis, Fzd9 is expressed in all
      spermatogenic cell types. Immunohistochemical studies of cells transfected with a
      Fzd9 expression construct confirm that Fzd9 is a membrane protein. These results 
      suggest potential Wnt ligands of Fzd9, a role of Fzd9 in skeletal muscle
      specification, and contributions of FZD9 to the WBS phenotype.
CI  - Copyright 1999 Academic Press.
FAU - Wang, Y K
AU  - Wang YK
AD  - Howard Hughes Medical Institute, Stanford University School of Medicine,
      Stanford, California 94305, USA.
FAU - Sporle, R
AU  - Sporle R
FAU - Paperna, T
AU  - Paperna T
FAU - Schughart, K
AU  - Schughart K
FAU - Francke, U
AU  - Francke U
LA  - eng
SI  - GENBANK/AF088850
GR  - HD33505/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (FZD3 protein, human)
RN  - 0 (Frizzled Receptors)
RN  - 0 (Fzd3 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Membrane/immunology/metabolism
MH  - Chromosome Mapping
MH  - DNA, Complementary/chemistry/genetics
MH  - Embryo, Mammalian/metabolism
MH  - Frizzled Receptors
MH  - Gene Deletion
MH  - Gene Expression
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nervous System/embryology/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cell Surface/*genetics/immunology
MH  - *Receptors, G-Protein-Coupled
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Somites/metabolism
MH  - Testis/embryology/metabolism
MH  - Tissue Distribution
MH  - Williams Syndrome/*genetics
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0888-7543(99)95773-7 [pii]
AID - 10.1006/geno.1999.5773 [doi]
PST - ppublish
SO  - Genomics. 1999 Apr 15;57(2):235-48. doi: 10.1006/geno.1999.5773.