PMID- 10198162
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20041117
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 57
IP  - 2
DP  - 1999 Apr 15
TI  - cDNA cloning and mapping of a novel subtype of glutamine:fructose-6-phosphate
      amidotransferase (GFAT2) in human and mouse.
PG  - 227-34
AB  - We subcloned human and mouse full-length cDNAs of a novel subtype of
      glutamine:fructose-6-phosphate amidotransferase (GFAT), which was designated
      GFAT2 (the previously reported GFAT was named GFAT1). Both the human and the
      mouse GFAT2 proteins deduced from their open reading frame sequences are composed
      of 682 amino acids of approximately 77.0 kDa. At the amino acid level, homologies
      between the human GFAT1 and GFAT2, between the mouse GFAT1 and GFAT2, and between
      the human GFAT2 and the mouse GFAT2 were 75.6, 74.7, and 97. 2%, respectively.
      Northern blot analysis using probe specific to human GFAT1 or GFAT2 showed that
      major transcripts were approximately 3.0 kb in both the human GFAT subtypes. The 
      analysis also revealed different tissue distribution between GFAT1 and GFAT2:
      GFAT1 was more highly expressed in the placenta, pancreas, and testis than GFAT2;
      GFAT2 was expressed throughout the central nervous system, especially in the
      spinal cord, but GFAT1 expression was weak. The locus was mapped to human
      chromosome 5q and mouse chromosome 11, where a synteny between the two species
      has been known. GFAT2 can provide insights into understanding the roles of the
      hexosamine pathway in various tissues, particularly with the development of
      glucose toxicity and diabetes complications.
CI  - Copyright 1999 Academic Press.
FAU - Oki, T
AU  - Oki T
AD  - Tsukuba Research Laboratories, Eisai Company, Ltd., 5-1-3, Tokodai, Tsukuba,
      Ibaraki, 300-2635, Japan.
FAU - Yamazaki, K
AU  - Yamazaki K
FAU - Kuromitsu, J
AU  - Kuromitsu J
FAU - Okada, M
AU  - Okada M
FAU - Tanaka, I
AU  - Tanaka I
LA  - eng
SI  - GENBANK/AB012608
SI  - GENBANK/AB012609
SI  - GENBANK/AB016778
SI  - GENBANK/AB016779
SI  - GENBANK/AB016780
SI  - GENBANK/AB016789
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.6.1.16 (Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing))
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/*genetics
MH  - Female
MH  - Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/*genetics
MH  - Humans
MH  - Isoenzymes/*genetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0888-7543(99)95785-3 [pii]
AID - 10.1006/geno.1999.5785 [doi]
PST - ppublish
SO  - Genomics. 1999 Apr 15;57(2):227-34. doi: 10.1006/geno.1999.5785.