PMID- 10198159
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20111014
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 57
IP  - 2
DP  - 1999 Apr 15
TI  - The Bax inhibitor-1 gene is differentially regulated in adult testis and
      developing lung by two alternative TATA-less promoters.
PG  - 201-8
AB  - We identified Bax inhibitor-1, BI-1, as a developmentally regulated gene product 
      in perinatal lung using suppressive subtractive hybridization. BI-1 is a novel
      suppressor of apoptosis that was previously cloned as testis-enhanced gene
      transcript (TEGT). However, sequence analysis of lung BI-1 revealed unique
      nucleotides starting 29 bases upstream of the ATG initiation codon and extending 
      to the 5' end of lung-derived BI-1 cDNA compared to the original transcript from 
      the testis. Cloning and sequencing of the upstream region of the BI-1 gene
      revealed that these unique sequences originated from two alternative first exons,
      located in tandem and separated by approximately 600 bases. Neither was preceded 
      by a TATA box in the usual position, and S1 nuclease mapping at each exon 1
      revealed multiple transcription start points with a major site being overlapped
      by a consensus initiator element. Promoter activity from each region was
      documented by transient transfection analysis in vitro using DNA sequences
      ligated to a reporter gene. The proximal promoter, P1, may exhibit cell
      type-specific differences in fibroblasts versus epithelia, whereas the distal
      promoter, P2, may exhibit species-specific differences in rat versus human cells.
      RT-PCR analysis for expression in adult tissues using exon 1-specific 5' primers 
      and common 3' primers revealed that P1 is tissue-specific; P2 is ubiquitously
      active. The developmental regulation of BI-1 in the late fetal and early
      postnatal lung is specific for P2, indicating that these two TATA-less promoters 
      are differentially regulated in adult testis and developing lung. Since Bax
      inhibitor-1 functions as a suppressor of apoptosis, its expression could provide 
      a survival advantage for select cell populations during the peak period of
      apoptosis that occurs at birth.
CI  - Copyright 1999 Academic Press.
FAU - Jean, J C
AU  - Jean JC
AD  - The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts
      02118, USA.
FAU - Oakes, S M
AU  - Oakes SM
FAU - Joyce-Brady, M
AU  - Joyce-Brady M
LA  - eng
SI  - GENBANK/AF118564
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TMBIM6 protein, human)
RN  - 0 (Tmbim6 protein, rat)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Blotting, Northern
MH  - Cell Line
MH  - DNA/chemistry/genetics
MH  - DNA, Complementary/genetics
MH  - Fetus/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - HeLa Cells
MH  - Humans
MH  - Lung/embryology/growth & development/*metabolism
MH  - Male
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - *Proteins
MH  - RNA/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - TATA Box
MH  - Testis/*metabolism
MH  - Tissue Distribution
MH  - Transcription, Genetic
EDAT- 1999/04/13 00:00
MHDA- 1999/04/13 00:01
CRDT- 1999/04/13 00:00
PHST- 1999/04/13 00:00 [pubmed]
PHST- 1999/04/13 00:01 [medline]
PHST- 1999/04/13 00:00 [entrez]
AID - S0888-7543(99)95761-0 [pii]
AID - 10.1006/geno.1999.5761 [doi]
PST - ppublish
SO  - Genomics. 1999 Apr 15;57(2):201-8. doi: 10.1006/geno.1999.5761.