PMID- 10198061
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 4
DP  - 1999 Apr
TI  - beta-catenin can be transported into the nucleus in a Ran-unassisted manner.
PG  - 1119-31
AB  - The nuclear accumulation of beta-catenin plays an important role in the
      Wingless/Wnt signaling pathway. This study describes an examination of the
      nuclear import of beta-catenin in living mammalian cells and in vitro semi-intact
      cells. When injected into the cell cytoplasm, beta-catenin rapidly migrated into 
      the nucleus in a temperature-dependent and wheat germ agglutinin-sensitive
      manner. In the cell-free import assay, beta-catenin rapidly migrates into the
      nucleus without the exogenous addition of cytosol, Ran, or ATP/GTP. Cytoplasmic
      injection of mutant Ran defective in its GTP hydrolysis did not prevent
      beta-catenin import. Studies using tsBN2, a temperature-sensitive mutant cell
      line that possesses a point mutation in the RCC1 gene, showed that the import of 
      beta-catenin is insensitive to nuclear Ran-GTP depletion. These results show that
      beta-catenin possesses the ability to constitutively translocate through the
      nuclear pores in a manner similar to importin beta in a Ran-unassisted manner. We
      further showed that beta-catenin also rapidly exits the nucleus in homokaryons,
      suggesting that the regulation of nuclear levels of beta-catenin involves both
      nuclear import and export of this molecule.
FAU - Yokoya, F
AU  - Yokoya F
AD  - Department of Anatomy and Cell Biology, Osaka University Medical School, Osaka
      565-0871, Japan. yyoneda@anat3.med.osaka-u.ac.jp
FAU - Imamoto, N
AU  - Imamoto N
FAU - Tachibana, T
AU  - Tachibana T
FAU - Yoneda, Y
AU  - Yoneda Y
LA  - eng
GR  - U17 CE002006/CE/NCIPC CDC HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Cadherins/metabolism
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Cytoskeletal Proteins/isolation & purification/*metabolism
MH  - Cytosol/metabolism
MH  - Dogs
MH  - Escherichia coli
MH  - GTP-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Karyopherins
MH  - Kidney
MH  - Kinetics
MH  - Mice
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/genetics/*metabolism
MH  - Recombinant Fusion Proteins/isolation & purification/metabolism
MH  - Temperature
MH  - *Trans-Activators
MH  - beta Catenin
MH  - ran GTP-Binding Protein
PMC - PMC25239
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1091/mbc.10.4.1119 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Apr;10(4):1119-31. doi: 10.1091/mbc.10.4.1119.