PMID- 10197984
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 7
DP  - 1999 Apr 1
TI  - Human step II splicing factor hSlu7 functions in restructuring the spliceosome
      between the catalytic steps of splicing.
PG  - 841-50
AB  - The spliceosome catalyzes pre-mRNA splicing in two steps. After catalytic step I,
      a major remodeling of the spliceosome occurs to establish the active site for
      step II. Here, we report the isolation of a cDNA encoding hSlu7, the human
      homolog of the yeast second step splicing factor Slu7. We show that hSlu7
      associates with the spliceosome late in the splicing pathway, but at a stage
      prior to recognition of the 3' splice site for step II. In the absence of hSlu7, 
      splicing is stalled between the catalytic steps in a novel complex, the
      CDeltahSlu7 complex. We provide evidence that this complex differs significantly 
      in structure from the known spliceosomal complexes, yet is a functional
      intermediate between the catalytic steps of splicing. Together, our observations 
      indicate that hSlu7 is required for a structural alteration of the spliceosome
      prior to the establishment of the catalytically active spliceosome for step II.
FAU - Chua, K
AU  - Chua K
AD  - Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115
      USA.
FAU - Reed, R
AU  - Reed R
LA  - eng
SI  - GENBANK/AF101074
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Fushi Tarazu Transcription Factors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA Splicing Factors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (SLU7 protein, human)
RN  - 0 (Tropomyosin)
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Amino Acid Sequence
MH  - Blotting, Western
MH  - Expressed Sequence Tags
MH  - Fushi Tarazu Transcription Factors
MH  - Globins/metabolism
MH  - HeLa Cells
MH  - Homeodomain Proteins/metabolism
MH  - Humans
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - RNA Splicing/*physiology
MH  - RNA Splicing Factors
MH  - RNA-Binding Proteins/*metabolism
MH  - *Ribonucleoproteins, Small Nuclear
MH  - Sequence Homology, Amino Acid
MH  - Spliceosomes/*metabolism
MH  - Time Factors
MH  - Tropomyosin/metabolism
PMC - PMC316594
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1101/gad.13.7.841 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Apr 1;13(7):841-50. doi: 10.1101/gad.13.7.841.