PMID- 10197766
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 88
IP  - 2
DP  - 1999 Jan
TI  - Connexin30 in rodent, cat and human brain: selective expression in gray matter
      astrocytes, co-localization with connexin43 at gap junctions and late
      developmental appearance.
PG  - 447-68
AB  - We previously presented evidence [Nagy et al. (1997) Neuroscience 78, 533-548]
      that, in addition to their ubiquitous expression of connexin43, astrocytes
      produce a second connexin suggested to be connexin30, a recently discovered
      member of the family of gap junction proteins. A connexin30 specific antibody was
      subsequently developed and utilized here to confirm and extend our earlier
      observations. On western blots, this antibody detected a 30,000 mol. wt protein
      in rat, mouse, cat and human brain, and exhibited no cross-reaction with
      connexin43, connexin26 or any other known connexins expressed in brain.
      Immunohistochemically, connexin30 was localized in astrocytes, at gap junctions
      between these cells and on the astrocyte side of gap junctions between astrocytes
      and oligodendrocytes. Double labelling revealed co-localization of connexin30 and
      connexin43 at astrocytic gap junctions. Punctate immunolabelling patterns for
      both connexins were qualitatively similar, but differences were evident. In
      contrast to regional connexin43 expression, diencephalic and hindbrain areas
      exhibited considerably greater expression than forebrain areas, subcortical
      perivascular astrocytic endfeet were more heavily labelled for connexin30, white 
      matter tracts such as corpus callosum, internal capsule and anterior commissure
      were devoid of connexin30, and appreciable levels of connexin30 during
      development were not seen until about postnatal day 15. These results indicate
      that connexin30 is expressed by gray, but not white matter astrocytes, its
      distribution is highly heterogeneous in gray matter, it is co-localized with
      connexin43 at astrocytic gap junctions where it forms homotypic or heterotypic
      junctions, and its emergence is delayed until relatively late during brain
      maturation. Taken together, these results suggest that astrocytic connexin30
      expression at both regional and cellular levels is subject to regulation in adult
      brain as well as during brain development.
FAU - Nagy, J I
AU  - Nagy JI
AD  - Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, 
      Canada.
FAU - Patel, D
AU  - Patel D
FAU - Ochalski, P A
AU  - Ochalski PA
FAU - Stelmack, G L
AU  - Stelmack GL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Connexin 30)
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (DFNA3 protein, human)
RN  - 0 (GJB6 protein, human)
RN  - 0 (Gjb6 protein, mouse)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Astrocytes/*chemistry/metabolism/ultrastructure
MH  - Blotting, Western
MH  - Brain/cytology/growth & development
MH  - Brain Chemistry
MH  - Cats
MH  - Cell Communication/physiology
MH  - Connexin 30
MH  - Connexin 43/*analysis/biosynthesis/immunology
MH  - Connexins/*analysis/biosynthesis/immunology
MH  - Fluorescent Antibody Technique
MH  - Gap Junctions/*chemistry
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
MH  - Oligodendroglia/chemistry/metabolism/ultrastructure
MH  - Peptide Fragments/chemistry/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Species Specificity
MH  - Spinal Cord/chemistry/cytology/growth & development
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - S0306452298001912 [pii]
AID - 10.1016/s0306-4522(98)00191-2 [doi]
PST - ppublish
SO  - Neuroscience. 1999 Jan;88(2):447-68. doi: 10.1016/s0306-4522(98)00191-2.