PMID- 10197531
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20191210
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 22
IP  - 3
DP  - 1999 Mar
TI  - EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane
      microdomains.
PG  - 511-24
AB  - Transmembrane ephrinB proteins have important functions during embryonic
      patterning as ligands for Eph receptor tyrosine kinases and presumably as
      signal-transducing receptor-like molecules. Consistent with "reverse" signaling, 
      ephrinB1 is localized in sphingo-lipid/cholesterol-enriched raft microdomains,
      platforms for the localized concentration and activation of signaling molecules. 
      Glutamate receptor-interacting protein (GRIP) and a highly related protein, which
      we have termed GRIP2, are recruited into these rafts through association with the
      C-terminal PDZ target site of ephrinB1. Stimulation of ephrinB1 with soluble
      EphB2 receptor ectodomain causes the formation of large raft patches that also
      contain GRIP proteins. Moreover, a GRIP-associated serine/threonine kinase
      activity is recruited into ephrinB1-GRIP complexes. Our findings suggest that
      GRIP proteins provide a scaffold for the assembly of a multiprotein signaling
      complex downstream of ephrinB ligands.
FAU - Bruckner, K
AU  - Bruckner K
AD  - Developmental Biology Programme, European Molecular Biology Laboratory,
      Heidelberg, Germany.
FAU - Pablo Labrador, J
AU  - Pablo Labrador J
FAU - Scheiffele, P
AU  - Scheiffele P
FAU - Herb, A
AU  - Herb A
FAU - Seeburg, P H
AU  - Seeburg PH
FAU - Klein, R
AU  - Klein R
LA  - eng
SI  - GENBANK/AF072509
SI  - GENBANK/AJ133439
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Ephrin-B1)
RN  - 0 (Grip1 protein, mouse)
RN  - 0 (Grip1 protein, rat)
RN  - 0 (Grip2 protein, mouse)
RN  - 0 (Grip2 protein, rat)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphoamino Acids)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Brain/embryology/metabolism
MH  - Carrier Proteins/genetics/metabolism/*physiology
MH  - Cell Line
MH  - Cell Membrane/metabolism/ultrastructure
MH  - Ephrin-B1
MH  - Gene Deletion
MH  - In Situ Hybridization
MH  - Intercellular Signaling Peptides and Proteins
MH  - Intracellular Signaling Peptides and Proteins
MH  - Ligands
MH  - Membrane Proteins/metabolism/*physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/metabolism/*physiology
MH  - Neurons/metabolism/ultrastructure
MH  - Organ Specificity
MH  - Phosphoamino Acids/metabolism
MH  - Precipitin Tests
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Rats
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - S0896-6273(00)80706-0 [pii]
AID - 10.1016/s0896-6273(00)80706-0 [doi]
PST - ppublish
SO  - Neuron. 1999 Mar;22(3):511-24. doi: 10.1016/s0896-6273(00)80706-0.