PMID- 10197430
OWN - NLM
STAT- MEDLINE
DCOM- 19990622
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 20
IP  - 2
DP  - 1999 Feb
TI  - muFKBP38: a novel murine immunophilin homolog differentially expressed in
      Schwannoma cells and central nervous system neurons in vivo.
PG  - 249-55
AB  - To better understand the process of multistage carcinogenesis in Schwann cells,
      we have attempted to isolate novel candidate genes involved in neoplastic
      progression of mouse malignant Schwannoma cells. The semi-differentiated
      Schwannoma cell line 56-24 and the less differentiated Schwannoma cell line 64-39
      were established from peripheral nerve sheath tumors arising in transgenic mice
      of the MBP/SV40 large T strain Tg29. By using the chemical cross-linking
      subtraction technique, we have cloned a novel murine cDNA that detects pronounced
      expression in 56-24 cells but not in 64-39 cells. The longest open reading frame 
      of the cDNA predicts a peptide showing 95% amino acid sequence homology to the
      recorded sequence of the human immunophilin homolog huFKBPr38, one of a family of
      proteins that are thought to interface with a wide range of intracellular signal 
      transduction systems. The predicted open reading frame of the corresponding gene,
      named muFKBP38, encodes a 38 kDa protein that harbors an FK-binding protein
      (FKBP) domain that is 36% identical to that of muFKBP52, a three-unit
      tetratricopeptide repeat and a consensus leucine-zipper repeat. Although muFKBP38
      mRNA was detected in both neurons and glial cells, pronounced expression of the
      immunophilin homolog appeared in various classes of neurons associated with the
      hippocampal formation, as shown by in situ hybridization analysis of adult mouse 
      brains. Taken together, these data indicate that muFKBP38 is (i) a novel
      potential marker for semi-differentiated Schwannomas, (ii) may form homomultimers
      and/or interact with other proteins, and (iii) may have a role in neurons
      associated with memory function.
FAU - Pedersen, K M
AU  - Pedersen KM
AD  - Department of Medical Biochemistry, University of Aarhus, Aarhus C, Denmark.
FAU - Finsen, B
AU  - Finsen B
FAU - Celis, J E
AU  - Celis JE
FAU - Jensen, N A
AU  - Jensen NA
LA  - eng
SI  - GENBANK/AF030635
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (DNA, Complementary)
RN  - 0 (Fkbp8 protein, mouse)
RN  - EC 5.2.1.- (Tacrolimus Binding Proteins)
RN  - EC 5.2.1.8 (Immunophilins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism/pathology
MH  - Central Nervous System/cytology
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunophilins/*genetics
MH  - In Situ Hybridization
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neurilemmoma/*genetics
MH  - Neurons/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - *Tacrolimus Binding Proteins
MH  - Tumor Cells, Cultured
EDAT- 1999/04/10 02:04
MHDA- 2000/08/12 11:00
CRDT- 1999/04/10 02:04
PHST- 1999/04/10 02:04 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/04/10 02:04 [entrez]
AID - 10.1002/(SICI)1522-2683(19990201)20:2<249::AID-ELPS249>3.0.CO;2-F [pii]
AID - 10.1002/(SICI)1522-2683(19990201)20:2<249::AID-ELPS249>3.0.CO;2-F [doi]
PST - ppublish
SO  - Electrophoresis. 1999 Feb;20(2):249-55. doi:
      10.1002/(SICI)1522-2683(19990201)20:2<249::AID-ELPS249>3.0.CO;2-F.