PMID- 10197429
OWN - NLM
STAT- MEDLINE
DCOM- 19990622
LR  - 20131121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 20
IP  - 2
DP  - 1999 Feb
TI  - Identification of true differentially expressed mRNAs in a pair of human bladder 
      transitional cell carcinomas using an improved differential display procedure.
PG  - 241-8
AB  - Differential display in combination with arbitrarily primed polymerase chain
      reaction (PCR) fingerprinting has become one of the most powerful techniques to
      identify and isolate mRNAs that are differentially expressed in pairs of
      biological samples. However, in many cases the cDNA band corresponding to the
      differentially amplified product contains several cDNA species that comigrate
      with the cDNA of interest due to the poor resolution of the fingerprinting gels, 
      thus hampering further analysis and identification of the desirable cDNA. To
      improve the electrophoretic resolution of differentially amplified cDNAs, we have
      utilized Resolver Gold agarose gel electrophoresis (Ingenius) as an additional
      step to overcome downstream problems encountered during RNA fingerprinting
      experiments. To illustrate the power of the modified differential display
      procedure we present a detailed analysis of the cDNA products differentially
      displayed in tumor biopsies obtained from a noninvasive (grade II, Ta) and an
      invasive (grade III, T2-T4) human bladder transitional cell carcinoma (TCC).
      Several genes that were differentially expressed in this tumor pair were
      identified. These included: tropomyosin 4, the protein disulfide isomerase
      precursor (PDI), MRP14, signal transducer CD24, keratins 8 and 13, cytochrome
      oxidase subunit IV (COXIV), putative transcription factor HOX-1.3, as well as two
      novel genes of yet unknown function. All of the identified cDNAs were shown to be
      truly differentially expressed by Northern blotting, reverse transcriptase-PCR
      (RT-PCR), and two-dimensional polyacrylamide gel electrophoresis (2-D PAGE)
      analysis of the corresponding lesions.
FAU - Gromova, I
AU  - Gromova I
AD  - Department of Medical Biochemistry and Danish Centre for Human Genome Research,
      The University of Aarhus, Aarhus C. ira@biobase.dk
FAU - Gromov, P
AU  - Gromov P
FAU - Celis, J E
AU  - Celis JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (DNA, Complementary)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - LHQ7J5KV9B (Bisbenzimidazole)
SB  - IM
MH  - Bisbenzimidazole
MH  - Carcinoma, Transitional Cell/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Electrophoresis, Agar Gel/*methods
MH  - Fluorescent Dyes
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genetic Heterogeneity
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger
MH  - RNA, Neoplasm/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1999/04/10 02:04
MHDA- 2000/08/12 11:00
CRDT- 1999/04/10 02:04
PHST- 1999/04/10 02:04 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/04/10 02:04 [entrez]
AID - 10.1002/(SICI)1522-2683(19990201)20:2<241::AID-ELPS241>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1522-2683(19990201)20:2<241::AID-ELPS241>3.0.CO;2-A [doi]
PST - ppublish
SO  - Electrophoresis. 1999 Feb;20(2):241-8. doi:
      10.1002/(SICI)1522-2683(19990201)20:2<241::AID-ELPS241>3.0.CO;2-A.