PMID- 10196478
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 230
IP  - 1
DP  - 1999 Apr 1
TI  - Molecular cloning and characterization of two sets of alpha-theta genes in the
      rat alpha-like globin gene cluster.
PG  - 91-9
AB  - The highly heterogeneous rat hemoglobin system was investigated at the gene
      level. Two regions of the alpha-like globin gene cluster from a Wistar rat were
      isolated. Four lambda Dash recombinant clones carrying rat alpha-like globin
      genes were localized on two distinct gene regions. A region of approximately 16kb
      was found to contain the 5'-IIalpha1-psi theta 1-3' loci, and another of
      approximately 24kb the 5'-IIalpha2-psi theta2-psiI alpha3-3' loci. Both IIalpha1 
      and IIalpha2 are considered to be active, coding the IIalpha-globin chain. The nt
      sequences of IIalpha1 and IIalpha2 are identical except for six nt in the
      non-coding region. The psiI alpha3 locus is a truncated pseudogene. The putative 
      promoter region of an alpha-like globin gene is joined directly to the third
      exon, homologous to that of Ialpha-globin cDNA. psi theta1 and psi theta2 are
      also pseudogenes, as evidenced by several deletions located in the protein-coding
      regions of these loci. The psi theta1 and psi theta2 loci exhibit extensive
      homology, but the restriction maps of these genes and their flanking regions
      differ considerably. Genomic Southern blot analyses of the total liver DNA from
      six rats showed the existence of three theta-globin-related genes, including psi 
      theta1 and psi theta2. These results indicate that the two gene regions
      investigated are not allelic variants, but may be generated by block duplication.
      This is the first report of the existence of rodent theta-globin genes.
FAU - Satoh, H
AU  - Satoh H
AD  - Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo
      113, Japan. hsatoh@biomol.sci.toho-u.ac.jp
FAU - Inokuchi, N
AU  - Inokuchi N
FAU - Nagae, Y
AU  - Nagae Y
FAU - Okazaki, T
AU  - Okazaki T
LA  - eng
SI  - GENBANK/X56324
SI  - GENBANK/X56325
SI  - GENBANK/X56326
SI  - GENBANK/X56327
SI  - GENBANK/X56328
SI  - GENBANK/X56329
SI  - GENBANK/X56330
SI  - GENBANK/X56331
SI  - GENBANK/X65495
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Evolution, Molecular
MH  - Gene Duplication
MH  - Globins/*genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Promoter Regions, Genetic
MH  - Pseudogenes/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Restriction Mapping
MH  - Sequence Alignment
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - S0378-1119(99)00055-4 [pii]
AID - 10.1016/s0378-1119(99)00055-4 [doi]
PST - ppublish
SO  - Gene. 1999 Apr 1;230(1):91-9. doi: 10.1016/s0378-1119(99)00055-4.