PMID- 10196368
OWN - NLM
STAT- MEDLINE
DCOM- 19990623
LR  - 20190513
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 8
IP  - 5
DP  - 1999 May
TI  - Imprinting of a RING zinc-finger encoding gene in the mouse chromosome region
      homologous to the Prader-Willi syndrome genetic region.
PG  - 795-803
AB  - A novel locus in the human Prader-Willi syndrome (PWS) region encodes the
      imprinted ZNF127 and antisense ZNF127AS genes. Here, we show that the mouse
      ZNF127 ortholog, Zfp127, encodes a homologous putative zinc-finger polypeptide,
      with a RING (C3HC4) and three C3H zinc-finger domains that suggest function as a 
      ribonucleoprotein. By the use of RT-PCR across an in-frame hexamer tandem repeat 
      and RNA from a Mus musculus x M.spretus F1interspecific cross, we show that
      Zfp127 is expressed only from the paternal allele in brain, heart and kidney.
      Similarly, Zfp127 is expressed in differentiated cells derived from androgenetic 
      embryonic stem cells and normal embryos but not those from parthogenetic
      embryonic stem cells. We hypothesize that the gametic imprint may be set, at
      least in part, by the transcriptional activity of Zfp127 in pre- and post-meiotic
      male germ cells. Therefore, Zfp127 is a novel imprinted gene that may play a role
      in the imprinted phenotype of mouse models of PWS.
FAU - Jong, M T
AU  - Jong MT
AD  - Department of Genetics, Case Western Reserve University School of Medicine and
      Center for Human Genetics, University Hospitals of Cleveland, 10900 Euclid
      Avenue, Cleveland, OH 44106-4955, USA.
FAU - Carey, A H
AU  - Carey AH
FAU - Caldwell, K A
AU  - Caldwell KA
FAU - Lau, M H
AU  - Lau MH
FAU - Handel, M A
AU  - Handel MA
FAU - Driscoll, D J
AU  - Driscoll DJ
FAU - Stewart, C L
AU  - Stewart CL
FAU - Rinchik, E M
AU  - Rinchik EM
FAU - Nicholls, R D
AU  - Nicholls RD
LA  - eng
GR  - HD34191/HD/NICHD NIH HHS/United States
GR  - HD36079/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (DNA, Antisense)
RN  - 0 (Mkrn3 protein, mouse)
RN  - 0 (Ribonucleoproteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Cloning, Molecular
MH  - DNA, Antisense
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - *Genomic Imprinting
MH  - Humans
MH  - Kidney/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Prader-Willi Syndrome/*genetics
MH  - Ribonucleoproteins/*genetics/metabolism
MH  - Spermatozoa/physiology
MH  - Testis/metabolism
MH  - Transcription, Genetic
MH  - Zinc Fingers/*genetics
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - ddc084 [pii]
AID - 10.1093/hmg/8.5.795 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1999 May;8(5):795-803. doi: 10.1093/hmg/8.5.795.