PMID- 10196349
OWN - NLM
STAT- MEDLINE
DCOM- 19990519
LR  - 20220402
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 5
DP  - 1999 May
TI  - A sodium-dependent neutral-amino-acid transporter mediates infections of feline
      and baboon endogenous retroviruses and simian type D retroviruses.
PG  - 4470-4
AB  - The type D simian retroviruses cause immunosuppression in macaques and have been 
      reported as a presumptive opportunistic infection in a patient with AIDS.
      Previous evidence based on viral interference has strongly suggested that the
      type D simian viruses share a common but unknown cell surface receptor with three
      type C viruses: feline endogenous virus (RD114), baboon endogenous virus, and
      avian reticuloendotheliosis virus. Furthermore, the receptor gene for these
      viruses has been mapped to human chromosome 19q13.1-13.2. We now report the
      isolation and characterization of a cell surface receptor for this group of
      retroviruses by using a human T-lymphocyte cDNA library in a retroviral vector.
      Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were
      transduced with the retroviral library and then challenged with an
      RD114-pseudotyped virus containing a dominant selectable gene for puromycin
      resistance. Puromycin selection yielded 12 cellular clones that were highly
      susceptible to a beta-galactosidase-encoding lacZ(RD114) pseudotype virus. Using 
      PCR primers specific for vector sequences, we amplified a common 2.9-kb product
      from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary
      cells conferred susceptibility to RD114, baboon endogenous virus, and the type D 
      simian retroviruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that 
      had 98% identity with the previously cloned human Na+-dependent
      neutral-amino-acid transporter Bo. Accordingly, expression of the RD114 receptor 
      in NIH 3T3 cells resulted in enhanced cellular uptake of L-[3H]alanine and
      L-[3H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor
      is widely expressed in human tissues and cell lines, including hematopoietic
      cells. The human Bo transporter gene has been previously mapped to 19q13.3, which
      is closely linked to the gene locus of the RD114 receptor.
FAU - Tailor, C S
AU  - Tailor CS
AD  - Department of Biochemistry and Molecular Biology, Oregon Health Sciences
      University, Portland, Oregon 97201-3098, USA. tailorc@ohsu.edu
FAU - Nouri, A
AU  - Nouri A
FAU - Zhao, Y
AU  - Zhao Y
FAU - Takeuchi, Y
AU  - Takeuchi Y
FAU - Kabat, D
AU  - Kabat D
LA  - eng
SI  - GENBANK/AF105423
GR  - WT_/Wellcome Trust/United Kingdom
GR  - CA25810/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Amino Acid Transport System ASC)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Virus)
RN  - 0 (SLC1A5 protein, human)
RN  - 0 (Slc1a5 protein, mouse)
RN  - 0 (Slc1a5 protein, rat)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - *Amino Acid Transport System ASC
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cats
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Endogenous Retroviruses/*metabolism/physiology
MH  - Gene Expression
MH  - Humans
MH  - Mice
MH  - Minor Histocompatibility Antigens
MH  - Molecular Sequence Data
MH  - Papio
MH  - Rats
MH  - Receptors, Virus/genetics/*metabolism
MH  - Retroviruses, Simian/*metabolism/physiology
MH  - Sodium/*metabolism
MH  - Tissue Distribution
PMC - PMC104338
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1128/JVI.73.5.4470-4474.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 May;73(5):4470-4. doi: 10.1128/JVI.73.5.4470-4474.1999.