PMID- 10196226
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 16
DP  - 1999 Apr 16
TI  - The small heat shock-related protein, HSP20, is phosphorylated on serine 16
      during cyclic nucleotide-dependent relaxation.
PG  - 11344-51
AB  - The small heat shock-related protein 20 (HSP20) is present in four isoforms in
      bovine carotid artery smooth muscles. Three of the isoforms are phosphorylated
      and one is not. Increases in the phosphorylation of two isoforms of HSP20
      (isoform 3, pI 5.9; and 8, pI 5.7) are associated with cyclic
      nucleotide-dependent relaxation of bovine carotid artery smooth muscles.
      Increases in the phosphorylation of another isoform (isoform 4, pI 6.0) are
      associated with phorbol ester-induced contraction of bovine carotid artery smooth
      muscles. In this investigation we determined that isoforms 3 and 8 are
      phosphorylated on Ser16 of the HSP20 molecule during activation of cAMP-dependent
      signaling pathways. Phosphorylation state-specific antibodies produced against a 
      peptide containing phosphorylated Ser16 recognized isoforms 3 and 8 but not
      isoform 4. In human vascular tissue, only isoform 3 is present. Incubation of
      transiently permeabilized strips of bovine carotid artery smooth muscle with
      synthetic peptides in which Ser16 is phosphorylated, inhibits contractile
      responses to high extracellular KCl and to serotonin. These data suggest that
      phosphorylation of HSP20 on Ser16 modulates cAMP-dependent vasorelaxation.
FAU - Beall, A
AU  - Beall A
AD  - Department of Medicine (Institute for Molecular Medicine and Genetics), Medical
      College of Georgia, Augusta, Georgia 30912, USA.
FAU - Bagwell, D
AU  - Bagwell D
FAU - Woodrum, D
AU  - Woodrum D
FAU - Stoming, T A
AU  - Stoming TA
FAU - Kato, K
AU  - Kato K
FAU - Suzuki, A
AU  - Suzuki A
FAU - Rasmussen, H
AU  - Rasmussen H
FAU - Brophy, C M
AU  - Brophy CM
LA  - eng
GR  - R01 HL58027-01/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (HSP20 Heat-Shock Proteins)
RN  - 0 (HSPB6 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Peptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 452VLY9402 (Serine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases)
SB  - IM
EIN - J Biol Chem 1999 Sep 24;274(39):28058
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cyclic AMP/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Cyclic GMP-Dependent Protein Kinases/metabolism
MH  - DNA Primers
MH  - HSP20 Heat-Shock Proteins
MH  - Heat-Shock Proteins/chemistry/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Muscle Contraction/drug effects
MH  - Muscle Relaxation/drug effects
MH  - Muscle, Smooth, Vascular/*metabolism
MH  - Peptides/pharmacology
MH  - Phosphoproteins/chemistry/*metabolism
MH  - Phosphorylation
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Serine/*metabolism
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1074/jbc.274.16.11344 [doi]
AID - S0021-9258(19)73645-X [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 16;274(16):11344-51. doi: 10.1074/jbc.274.16.11344.