PMID- 10196175
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 16
DP  - 1999 Apr 16
TI  - Alternative splicing determines the intracellular localization of the novel
      nuclear protein Nop30 and its interaction with the splicing factor SRp30c.
PG  - 10951-62
AB  - We report on the molecular cloning of a novel human cDNA by its interaction with 
      the splicing factor SRp30c in a yeast two-hybrid screen. This cDNA is
      predominantly expressed in muscle and encodes a protein that is present in the
      nucleoplasm and concentrated in nucleoli. It was therefore termed Nop30
      (nucleolar protein of 30 kDa). We have also identified a related cDNA with a
      different carboxyl terminus. Sequencing of the NOP gene demonstrated that both
      cDNAs are generated by alternative 5' splice site usage from a single gene that
      consists of four exons, spans at least 1800 nucleotides, and is located on
      chromosome 16q21-q23. The alternative 5' splice site usage introduces a
      frameshift creating two different carboxyl termini. The carboxyl terminus of
      Nop30 is rich in serines and arginines and has been found to target the protein
      into the nucleus, whereas its isoform is characterized by proline/glutamic acid
      dipeptides in its carboxyl terminus and is predominantly found in the cytosol.
      Interaction studies in yeast, in vitro protein interaction assays, and
      co-immunoprecipitations demonstrated that Nop30 multimerizes and binds to the RS 
      domain of SRp30c but not to other splicing factors tested. Overexpression of
      Nop30 changes alternative exon usage in preprotachykinin and SRp20 reporter
      genes, suggesting that Nop30 influences alternative splice site selection in
      vivo.
FAU - Stoss, O
AU  - Stoss O
AD  - Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried,
      Germany.
FAU - Schwaiger, F W
AU  - Schwaiger FW
FAU - Cooper, T A
AU  - Cooper TA
FAU - Stamm, S
AU  - Stamm S
LA  - eng
SI  - GENBANK/AF064598
SI  - GENBANK/AF064599
SI  - GENBANK/AF064600
GR  - HL45565/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Muscle Proteins)
RN  - 0 (NOL3 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 16
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Muscle Proteins
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins
MH  - Serine-Arginine Splicing Factors
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1074/jbc.274.16.10951 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 16;274(16):10951-62. doi: 10.1074/jbc.274.16.10951.