PMID- 10196146
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 16
DP  - 1999 Apr 16
TI  - Specific isoforms of actin-binding proteins on distinct populations of
      Golgi-derived vesicles.
PG  - 10743-50
AB  - Golgi membranes and Golgi-derived vesicles are associated with multiple
      cytoskeletal proteins and motors, the diversity and distribution of which have
      not yet been defined. Carrier vesicles were separated from Golgi membranes, using
      an in vitro budding assay, and different populations of vesicles were separated
      using sucrose density gradients. Three main populations of vesicles labeled with 
      beta-COP, gamma-adaptin, or p200/myosin II were separated and analyzed for the
      presence of actin/actin-binding proteins. beta-Actin was bound to Golgi cisternae
      and to all populations of newly budded vesicles. Centractin was selectively
      associated with vesicles co-distributing with beta-COP-vesicles, while
      p200/myosin II (non-muscle myosin IIA) and non-muscle myosin IIB were found on
      different vesicle populations. Isoforms of the Tm5 tropomyosins were found on
      selected Golgi-derived vesicles, while other Tm isoforms did not colocalize with 
      Tm5 indicating the association of specialized actin filaments with Golgi-derived 
      vesicles. Golgi-derived vesicles were shown to bind to F-actin polymerized from
      cytosol with Jasplakinolide. Thus, newly budded, coated vesicles derived from
      Golgi membranes can bind to actin and are customized for differential
      interactions with microfilaments by the presence of selective arrays of
      actin-binding proteins.
FAU - Heimann, K
AU  - Heimann K
AD  - Centre for Molecular and Cellular Biology, University of Queensland, Brisbane,
      Queensland 4072, Australia.
FAU - Percival, J M
AU  - Percival JM
FAU - Weinberger, R
AU  - Weinberger R
FAU - Gunning, P
AU  - Gunning P
FAU - Stow, J L
AU  - Stow JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Actins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Protein Isoforms)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Golgi Apparatus/*metabolism
MH  - Microfilament Proteins/*metabolism
MH  - Myosins/metabolism
MH  - Protein Isoforms/*metabolism
MH  - Rats
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1074/jbc.274.16.10743 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 16;274(16):10743-50. doi: 10.1074/jbc.274.16.10743.