PMID- 10196145
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 16
DP  - 1999 Apr 16
TI  - mRNAs encoding a von Ebner's-like protein and the Huntington disease protein are 
      induced in rat male germ cells by Sertoli cells.
PG  - 10737-42
AB  - The success of spermatogenesis is dependent upon closely coordinated interactions
      between Sertoli cells and germ cells. To identify specific molecules that mediate
      interactions between somatic cells and germ cells in the rat testis, Sertoli
      cell-germ cell co-cultures and mRNA differential display were used. Two cDNAs,
      clone 1 (660 nucleotides) and clone 2 (390 nucleotides) were up-regulated when
      Sertoli cells were co-cultured with pachytene spermatocytes or round spermatids. 
      Northern blot analyses confirmed the differential display expression patterns.
      Sequence analyses indicated that clone 1 was similar to a von Ebner's gland
      protein (87% at the nucleotide level and 80% at the amino acid level) and clone 2
      was identical to a region of the Huntington disease protein. The von Ebner's-like
      protein mRNA was induced after 4 h of co-culture, while the Huntington disease
      protein required 18 h of co-culture for expression. The von Ebner's-like protein 
      was induced in germ cells by a secreted Sertoli cell factor(s) smaller than 10
      kDa that is sensitive to freezing and thawing or boiling. The Huntington disease 
      protein was induced in germ cells by a Sertoli cell secreted factor(s) larger
      than 10 kDa which survives freezing and thawing, but is inactivated by boiling.
FAU - Syed, V
AU  - Syed V
AD  - Center for Research on Reproduction and Women's Health and Department of
      Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, Pennsylvania
      19104, USA.
FAU - Gomez, E
AU  - Gomez E
FAU - Hecht, N B
AU  - Hecht NB
LA  - eng
SI  - GENBANK/AF123454
GR  - HD-11878/HD/NICHD NIH HHS/United States
GR  - T32HD07305/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Htt protein, rat)
RN  - 0 (Huntingtin Protein)
RN  - 0 (Lipocalin 1)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vegp1 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Coculture Techniques
MH  - Gene Expression Regulation
MH  - Huntingtin Protein
MH  - Huntington Disease/genetics
MH  - Lipocalin 1
MH  - Male
MH  - Meiosis
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Nuclear Proteins/*genetics
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Homology, Amino Acid
MH  - Sertoli Cells/cytology/*metabolism
MH  - Testis/cytology/*metabolism
MH  - Up-Regulation
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - 10.1074/jbc.274.16.10737 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 16;274(16):10737-42. doi: 10.1074/jbc.274.16.10737.