PMID- 10191308
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20191023
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 8
DP  - 1999 Apr 15
TI  - Functional analysis of a mouse brain Elk-type K+ channel.
PG  - 2906-18
AB  - Members of the Ether a go-go (Eag) K+ channel subfamilies Eag, Erg, and Elk are
      widely expressed in the nervous system, but their neural functions in vivo remain
      largely unknown. The biophysical properties of channels from the Eag and Erg
      subfamilies have been described, and based on their characteristic features and
      expression patterns, Erg channels have been associated with native currents in
      the heart. Little is known about the properties of channels from the Elk
      subfamily. We have identified a mouse gene, Melk2, that encodes a predicted
      polypeptide with 48% amino acid identity to Drosophila Elk but only 40 and 36%
      identity with mouse Erg (Merg) and Eag (Meag), respectively. Melk2 RNA appears to
      be expressed at high levels only in brain tissue. Functional expression of Melk2 
      in Xenopus oocytes reveals large, transient peaks of current at the onset of
      depolarization. Like Meag currents, Melk2 currents activate relatively quickly,
      but they lack the nonsuperimposable Cole-Moore shift characteristic of the Eag
      subfamily. Melk2 currents are insensitive to E-4031, a class III antiarrhythmic
      compound that blocks the Human Ether-a-go-go-Related Gene (HERG) channel and its 
      counterpart in native tissues, IKr. Melk2 channels exhibit inward rectification
      because of a fast C-type inactivation mechanism, but the slower rate of
      inactivation and the faster rate of activation results in less inward
      rectification than that observed in HERG channels. This characterization of Melk 
      currents should aid in identification of native counterparts to the Elk subfamily
      of channels in the nervous system.
FAU - Trudeau, M C
AU  - Trudeau MC
AD  - Department of Physiology, University of Wisconsin-Madison Medical School,
      Madison, Wisconsin 53706, USA.
FAU - Titus, S A
AU  - Titus SA
FAU - Branchaw, J L
AU  - Branchaw JL
FAU - Ganetzky, B
AU  - Ganetzky B
FAU - Robertson, G A
AU  - Robertson GA
LA  - eng
SI  - GENBANK/AF109143
GR  - R01 HL055973/HL/NHLBI NIH HHS/United States
GR  - R01 NS015390/NS/NINDS NIH HHS/United States
GR  - NS15390/NS/NINDS NIH HHS/United States
GR  - HL55973/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Ether-A-Go-Go Potassium Channels)
RN  - 0 (Kcnh3 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Potassium Channels)
RN  - EC 2.7.1.- (Melk protein, mouse)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Action Potentials/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/*physiology
MH  - Ether-A-Go-Go Potassium Channels
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Nerve Tissue Proteins/*physiology
MH  - Oocytes/physiology
MH  - Potassium Channels/genetics/*physiology
MH  - Protein-Serine-Threonine Kinases/*physiology
MH  - Sequence Homology, Amino Acid
MH  - Xenopus
PMC - PMC6782280
EDAT- 1999/04/07 00:00
MHDA- 1999/04/07 00:01
CRDT- 1999/04/07 00:00
PHST- 1999/04/07 00:00 [pubmed]
PHST- 1999/04/07 00:01 [medline]
PHST- 1999/04/07 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Apr 15;19(8):2906-18.