PMID- 10191299
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20071114
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 40
IP  - 4
DP  - 1999 Apr
TI  - Antipeptide antibodies reveal interrelationships of MBP 200 and MBP 235: unique
      apoB-specific receptors for triglyceride-rich lipoproteins on human
      monocyte-macrophages.
PG  - 744-52
AB  - Two human monocyte-macrophage (HMM) membrane binding proteins, (MBP) 200 and 235,
      are receptor candidates that bind to the apolipoprotein (apo)B-48 domain in
      triglyceride-rich lipoproteins for uptake independent of apoE. Microsequence
      analysis of the purified reduced MBP 200R characterized tryptic peptides of MBP
      200R. A synthetic peptide mimicking a unique, unambiguous 10-residue sequence
      (AEGLMVTGGR) induced antipeptide antibodies that specifically recognized MBP 200,
      235 and 200R, in 1- and 2-dimensional analyses, indicating 1) the ligand binding 
      protein was sequenced and 2) MBP 200 and 235 yielded MBP 200R upon reduction.
      These antibodies identified the MBPs in human blood-borne, THP-1, U937 MMs, and
      endothelial cells (EC) but not in human fibroblasts or Chinese hamster ovary
      (CHO) cells. Fluorescence activated cell sorting (FACS) analysis located the MBPs
      on the MM surface as necessary for receptor function. The 10-residue, unambiguous
      MBP 200-derived sequence is unique, with no matches in extant protein databases. 
      Antipeptide antibodies bind to the MBPs in reticuloendothelial cells that have
      this receptor activity, but not to proteins in cells that lack this receptor
      activity. These studies provide the first direct protein sequence and
      immunochemical data that a new, unique apoB receptor for triglyceride-rich
      lipoproteins exists in human monocytes, macrophages, and endothelial cells.
FAU - Bradley, W A
AU  - Bradley WA
AD  - Department of Medicine, Division of Gerontology and Geriatric Medicine,
      University of Alabama at Birmingham, Birmingham, AL 35294, USA.
FAU - Brown, M L
AU  - Brown ML
FAU - Ramprasad, M P
AU  - Ramprasad MP
FAU - Li, R
AU  - Li R
FAU - Song, R
AU  - Song R
FAU - Gianturco, S H
AU  - Gianturco SH
LA  - eng
GR  - HL44480/HL/NHLBI NIH HHS/United States
GR  - RR00032/RR/NCRR NIH HHS/United States
GR  - T32 HL-07703/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Antibodies)
RN  - 0 (Apolipoprotein B-48)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Epitopes)
RN  - 0 (Lipoproteins)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (Triglycerides)
RN  - 0 (apolipoprotein B receptor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/immunology
MH  - Antibody Specificity
MH  - Apolipoprotein B-48
MH  - Apolipoproteins B/metabolism
MH  - CHO Cells/chemistry
MH  - Cricetinae
MH  - Epitopes/immunology
MH  - Fibroblasts/chemistry
MH  - Flow Cytometry
MH  - Humans
MH  - Lipoproteins/*metabolism
MH  - Macrophages/*chemistry
MH  - Monocytes/*chemistry
MH  - Peptides/immunology
MH  - Receptors, Lipoprotein/*analysis/chemistry/metabolism
MH  - Sequence Analysis
MH  - Triglycerides/*analysis
EDAT- 1999/04/07 00:00
MHDA- 1999/04/07 00:01
CRDT- 1999/04/07 00:00
PHST- 1999/04/07 00:00 [pubmed]
PHST- 1999/04/07 00:01 [medline]
PHST- 1999/04/07 00:00 [entrez]
PST - ppublish
SO  - J Lipid Res. 1999 Apr;40(4):744-52.