PMID- 10191093
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20170922
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 57
IP  - 1
DP  - 1999 Apr 1
TI  - Cloning and characterization of NEU2, a human gene homologous to rodent soluble
      sialidases.
PG  - 137-43
AB  - Sialidases (EC 3.2.1.18) are a group of glycohydrolytic enzymes, widely
      distributed in nature, that cleave sialic acid residues from the oligosaccharide 
      components of glycoconjugates. All of the sialidase enzymes thus far
      characterized share an Asp block, repeated three to five times in the primary
      structure, and an F/YRIP sequence motif that is part of the active site. Using a 
      sequence homology-based approach, we have identified a novel human gene, named
      NEU2, mapping to chromosome 2q37. The nucleotide sequence analysis of the gene
      has shown that it contains only one intron of about 1.25 kb, and the longest open
      reading frame encodes a protein of 380 amino acids, with a two-Asp block
      consensus, and the YRIP sequence. In the putative promoter sequence there are a
      classical TATAA box and four E boxes, which are consensus binding sites for
      muscle-specific transcription factors. Northern blot analysis revealed expression
      of the NEU2 transcript only in skeletal muscle.
CI  - Copyright 1999 Academic Press.
FAU - Monti, E
AU  - Monti E
AD  - Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical
      Science Park, Milan, Italy.
FAU - Preti, A
AU  - Preti A
FAU - Rossi, E
AU  - Rossi E
FAU - Ballabio, A
AU  - Ballabio A
FAU - Borsani, G
AU  - Borsani G
LA  - eng
SI  - GENBANK/Y16535
GR  - TGM06S01/Telethon/Italy
GR  - TGM97000/Telethon/Italy
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - EC 3.2.1.18 (NEU2 protein, human)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosomes, Human, Pair 2
MH  - Databases, Factual
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Neuraminidase/*genetics
MH  - Physical Chromosome Mapping
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1999/04/07 00:00
MHDA- 1999/04/07 00:01
CRDT- 1999/04/07 00:00
PHST- 1999/04/07 00:00 [pubmed]
PHST- 1999/04/07 00:01 [medline]
PHST- 1999/04/07 00:00 [entrez]
AID - S0888-7543(99)95749-X [pii]
AID - 10.1006/geno.1999.5749 [doi]
PST - ppublish
SO  - Genomics. 1999 Apr 1;57(1):137-43. doi: 10.1006/geno.1999.5749.