PMID- 10187853
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Cell surface retention sequence binding protein-1 interacts with the v-sis gene
      product and platelet-derived growth factor beta-type receptor in simian sarcoma
      virus-transformed cells.
PG  - 10582-9
AB  - The cell surface retention sequence (CRS) binding protein-1 (CRSBP-1) is a newly 
      identified membrane glycoprotein which is hypothesized to be responsible for cell
      surface retention of the oncogene v-sis and c-sis gene products and other
      secretory proteins containing CRSs. In simian sarcoma virus-transformed NIH 3T3
      cells (SSV-NIH 3T3 cells), a fraction of CRSBP-1 was demonstrated at the cell
      surface and underwent internalization/recycling as revealed by cell surface 125I 
      labeling and its resistance/sensitivity to trypsin digestion. However, the
      majority of CRSBP-1 was localized in intracellular compartments as evidenced by
      the resistance of most of the 35S-metabolically labeled CRSBP-1 to trypsin
      digestion, and by indirect immunofluorescent staining. CRSBP-1 appeared to form
      complexes with proteolytically processed forms (generated at and/or after the
      trans-Golgi network) of the v-sis gene product and with a approximately 140-kDa
      proteolytically cleaved form of the platelet-derived growth factor (PDGF)
      beta-type receptor, as demonstrated by metabolic labeling and
      co-immunoprecipitation. CRSBP-1, like the v-sis gene product and PDGF beta-type
      receptor, underwent rapid turnover which was blocked in the presence of 100
      microM suramin. In normal and other transformed NIH 3T3 cells, CRSBP-1 was
      relatively stable and did not undergo rapid turnover and
      internalization/recycling at the cell surface. These results suggest that in
      SSV-NIH 3T3 cells, CRSBP-1 interacts with and forms ternary and binary complexes 
      with the newly synthesized v-sis gene product and PDGF beta-type receptor at the 
      trans-Golgi network and that the stable binary (CRSBP-1.v-sis gene product)
      complex is transported to the cell surface where it presents the v-sis gene
      product to unoccupied PDGF beta-type receptors during internalization/recycling.
FAU - Boensch, C
AU  - Boensch C
AD  - Department of Biochemistry and Molecular Biology, St. Louis University School of 
      Medicine, St. Louis, Missouri 63104, USA.
FAU - Huang, S S
AU  - Huang SS
FAU - Connolly, D T
AU  - Connolly DT
FAU - Huang, J S
AU  - Huang JS
LA  - eng
GR  - CA38808/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Membrane Proteins)
RN  - 0 (Oncogene Proteins v-sis)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (cell-surface retention-binding protein 1)
RN  - 6032D45BEM (Suramin)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - *Cell Transformation, Viral
MH  - Fluorescent Antibody Technique, Indirect
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Oncogene Proteins v-sis
MH  - Rabbits
MH  - Receptor, Platelet-Derived Growth Factor beta
MH  - Receptors, Platelet-Derived Growth Factor/*metabolism
MH  - Retroviridae Proteins, Oncogenic/*metabolism
MH  - Sarcoma Virus, Woolly Monkey/*metabolism
MH  - Suramin/metabolism
MH  - Surface Properties
MH  - Trypsin/metabolism
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10582 [doi]
AID - S0021-9258(19)73751-X [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10582-9. doi: 10.1074/jbc.274.15.10582.