PMID- 10187827
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Repression of human fibroblast growth factor 2 by a novel transcription factor.
PG  - 10382-7
AB  - Here we describe the cloning of the regulator of fibroblast growth factor 2
      (FGF-2) transcription (RFT) using a yeast one-hybrid screening with a defined
      motif in FGF-2 promoter as a target sequence. Overexpression of human RFT (RFT-A)
      reduces FGF-2 RNA and protein levels in both normal and tumor cell lines. Its
      splice variants, RFT-A' and RFT-B, have deletions in the putative DNA binding
      domain and fail to bind FGF-2 promoter and repress FGF-2 gene expression. The
      ratios of RFT isoforms differ between normal and tumor cells, with the splice
      variants dominating in tumor cells. Overexpression of RFT-A induces glioma cell
      death. Our data suggest that regulation of FGF-2 by RFT is important for cellular
      functions and may be impaired in certain tumors.
FAU - Ueba, T
AU  - Ueba T
AD  - Salk Institute for Biological Studies, La Jolla, California 92037, USA.
FAU - Kaspar, B
AU  - Kaspar B
FAU - Zhao, X
AU  - Zhao X
FAU - Gage, F H
AU  - Gage FH
LA  - eng
GR  - N01-NS-6-23438/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MBD1 protein, human)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA/metabolism
MH  - *DNA-Binding Proteins
MH  - Fibroblast Growth Factor 2/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Repressor Proteins/*chemistry/*genetics
MH  - Transcription Factors
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10382 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10382-7. doi: 10.1074/jbc.274.15.10382.