PMID- 10187817
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Molecular cloning and characterization of two novel pro-apoptotic isoforms of
      caspase-10.
PG  - 10301-8
AB  - Caspase-10/a (Mch4) and caspase-10/b (FLICE2) are related death effector
      domain-containing cysteine aspartases presumed to be at or near the apex of
      apoptotic signaling pathways. We report the cloning and characterization of two
      novel proteins that are splice isoforms of the caspase-10 family. Caspase-10/c is
      a truncated protein that is essentially a prodomain-only form of the caspase that
      lacks proteolytic activity in vitro but efficiently induces the formation of
      perinuclear filamentous structures and cell death in vivo. Caspase-10/c mRNA is
      specifically up-regulated upon TNF stimulation, suggesting a potential role of
      this isoform in amplifying the apoptotic response to extracellular stimuli such
      as cytokines. Caspase-10/d is a hybrid of the known caspases Mch4 and FLICE2, as 
      it is identical to FLICE2 except for the small (p12) catalytic subunit, which is 
      identical to Mch4. Caspase-10/d is proteolytically active in vitro and also
      induces cell death in vivo, although it is less active than Mch4. The mRNAs for
      all known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, 
      and skeletal muscle but are very poorly expressed or absent in these tissues in
      the adult, implying a possible role for the caspase-10 family in fetal
      development.
FAU - Ng, P W
AU  - Ng PW
AD  - Institute of Molecular and Cell Biology, National University of Singapore,
      Singapore 117609, Republic of Singapore.
FAU - Porter, A G
AU  - Porter AG
FAU - Janicke, R U
AU  - Janicke RU
LA  - eng
SI  - GENBANK/AF111344
SI  - GENBANK/AF111345
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.22.- (Caspase 10)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (interleukin 1beta-converting enzyme 2)
RN  - EC 3.4.22.63 (CASP10 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Apoptosis
MH  - Caspase 10
MH  - Caspases/chemistry/*genetics
MH  - Catalytic Domain
MH  - Cloning, Molecular
MH  - Humans
MH  - Isoenzymes/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10301 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10301-8. doi: 10.1074/jbc.274.15.10301.