PMID- 10187815
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - mE10, a novel caspase recruitment domain-containing proapoptotic molecule.
PG  - 10287-92
AB  - Apoptotic signaling is mediated by homophilic interactions between conserved
      domains present in components of the death pathway. The death domain, death
      effector domain, and caspase recruitment domain (CARD) are examples of such
      interaction motifs. We have identified a novel mammalian CARD-containing adaptor 
      molecule termed mE10 (mammalian E10). The N-terminal CARD of mE10 exhibits
      significant homology (47% identity and 64% similarity) to the CARD of a gene from
      Equine Herpesvirus type 2. The C-terminal region is unique. Overexpression of
      mE10 in MCF-7 human breast carcinoma cells induces apoptosis. Mutational analysis
      indicates that CARD-mediated mE10 oligomerization is essential for killing
      activity. The C terminus of mE10 bound to the zymogen form of caspase-9 and
      promoted its processing to the active dimeric species. Taken together, these data
      suggest a model where autoproteolytic activation of pro-caspase-9 is mediated by 
      mE10-induced oligomerization.
FAU - Yan, M
AU  - Yan M
AD  - Department of Molecular Oncology, Genentech, Inc., South San Francisco,
      California 94080, USA.
FAU - Lee, J
AU  - Lee J
FAU - Schilbach, S
AU  - Schilbach S
FAU - Goddard, A
AU  - Goddard A
FAU - Dixit, V
AU  - Dixit V
LA  - eng
SI  - GENBANK/AA119259
SI  - GENBANK/AA455396
SI  - GENBANK/AF127386
SI  - GENBANK/AF127387
SI  - GENBANK/W47752
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (APAF1 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apaf1 protein, mouse)
RN  - 0 (Apoptotic Protease-Activating Factor 1)
RN  - 0 (B-Cell CLL-Lymphoma 10 Protein)
RN  - 0 (BCL10 protein, human)
RN  - 0 (Bcl10 protein, mouse)
RN  - 0 (Carrier Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (CASP9 protein, human)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Casp9 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Apoptosis
MH  - Apoptotic Protease-Activating Factor 1
MH  - B-Cell CLL-Lymphoma 10 Protein
MH  - Binding Sites
MH  - Carrier Proteins/genetics/*metabolism
MH  - Caspase 3
MH  - Caspase 9
MH  - Caspases/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Proteins/metabolism
MH  - Sequence Alignment
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10287 [doi]
AID - S0021-9258(19)73713-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10287-92. doi: 10.1074/jbc.274.15.10287.