PMID- 10187812
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte
      development.
PG  - 10259-67
AB  - A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library 
      made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6
      kappa promoter kappaY element as a bait. The novel Ets protein was most closely
      related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore
      named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein
      family, as it differed significantly from Spi-B and PU.1 within helix 1 of the
      Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome
      10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by
      methylation interference, band-shift and site selection analysis, and activated
      transcription of a kappaY element reporter gene upon co-transfection of HeLa
      cells. Spi-C RNA was expressed in mature B lymphocytes and at lower levels in
      macrophages. Furthermore, pre-B cell and plasma cell lines were Spi-C-negative,
      suggesting that Spi-C might be a regulatory molecule during a specific phase of B
      lymphoid development.
FAU - Bemark, M
AU  - Bemark M
AD  - Immunology Unit, Department of Cell and Molecular Biology, Lund University, P. O.
      Box 7031, S-220 07 Lund, Sweden.
FAU - Martensson, A
AU  - Martensson A
FAU - Liberg, D
AU  - Liberg D
FAU - Leanderson, T
AU  - Leanderson T
LA  - eng
SI  - GENBANK/AF098863
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Immunoglobulin kappa-Chains)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (proto-oncogene protein Spi-1)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (RNA polymerase SP6)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - B-Lymphocytes/*cytology/metabolism
MH  - Cell Differentiation
MH  - Cloning, Molecular
MH  - DNA/metabolism
MH  - DNA-Directed RNA Polymerases/*metabolism
MH  - Down-Regulation
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Immunoglobulin kappa-Chains/*genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins/metabolism
MH  - Spleen/cytology/drug effects
MH  - Trans-Activators/*chemistry/*genetics/isolation & purification/metabolism
MH  - Transcriptional Activation
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10259 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10259-67. doi: 10.1074/jbc.274.15.10259.