PMID- 10187805
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Inhibition of receptor internalization by monodansylcadaverine selectively blocks
      p55 tumor necrosis factor receptor death domain signaling.
PG  - 10203-12
AB  - The 55-kDa receptor for tumor necrosis factor (TR55) triggers multiple signaling 
      cascades initiated by adapter proteins like TRADD and FAN. By use of the primary 
      amine monodansylcadaverine (MDC), we addressed the functional role of tumor
      necrosis factor (TNF) receptor internalization for intracellular signal
      distribution. We show that MDC does not prevent the interaction of the p55 TNF
      receptor (TR55) with FAN and TRADD. Furthermore, the activation of
      plasmamembrane-associated neutral sphingomyelinase activation as well as the
      stimulation of proline-directed protein kinases were not affected in MDC-treated 
      cells. In contrast, activation of signaling enzymes that are linked to the "death
      domain" of TR55, like acid sphingomyelinase and c-Jun-N-terminal protein kinase
      as well as TNF signaling of apoptosis in U937 and L929 cells, are blocked in the 
      presence of MDC. The results of our study suggest a role of TR55 internalization 
      for the activation of select TR55 death domain signaling pathways including those
      leading to apoptosis.
FAU - Schutze, S
AU  - Schutze S
AD  - Institute of Immunology, University of Kiel, 24105 Kiel, Germany.
      schuetze@immunologie.uni-kiel.de
FAU - Machleidt, T
AU  - Machleidt T
FAU - Adam, D
AU  - Adam D
FAU - Schwandner, R
AU  - Schwandner R
FAU - Wiegmann, K
AU  - Wiegmann K
FAU - Kruse, M L
AU  - Kruse ML
FAU - Heinrich, M
AU  - Heinrich M
FAU - Wickel, M
AU  - Wickel M
FAU - Kronke, M
AU  - Kronke M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (Ceramides)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NSMAF protein, human)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (TNF Receptor-Associated Factor 1)
RN  - 0 (fas Receptor)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
RN  - I9N81SC5HD (monodansylcadaverine)
RN  - L90BEN6OLL (Cadaverine)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - *Apoptosis
MH  - Cadaverine/*analogs & derivatives/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Ceramides/metabolism
MH  - Endocytosis
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Jurkat Cells
MH  - *Mitogen-Activated Protein Kinases
MH  - Potassium/metabolism
MH  - Proteins/metabolism
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - *Signal Transduction
MH  - Sphingomyelin Phosphodiesterase/metabolism
MH  - TNF Receptor-Associated Factor 1
MH  - U937 Cells
MH  - fas Receptor/immunology/metabolism
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10203 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10203-12. doi: 10.1074/jbc.274.15.10203.