PMID- 10187799
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - The mouse p97 (CDC48) gene. Genomic structure, definition of transcriptional
      regulatory sequences, gene expression, and characterization of a pseudogene.
PG  - 10154-62
AB  - Here we present the first description of the genomic organization,
      transcriptional regulatory sequences, and adult and embryonic gene expression for
      the mouse p97(CDC48) AAA ATPase. Clones representing two distinct p97 genes were 
      isolated in a genomic library screen, one of them likely representing a
      non-functional processed pseudogene. The coding region of the gene encoding the
      functional mRNA is interrupted by 16 introns and encompasses 20.4 kilobase pairs.
      Definition of the transcriptional initiation site and sequence analysis showed
      that the gene contains a TATA-less, GC-rich promoter region with an initiator
      element spanning the transcription start site. Cis-acting elements necessary for 
      basal transcription activity reside within 410 base pairs of the flanking region 
      as determined by transient transfection assays. In immunohistological analyses,
      p97 was widely expressed in embryos and adults, but protein levels were tightly
      controlled in a cell type- and cell differentiation-dependent manner. A
      remarkable heterogeneity in p97 immunostaining was found on a cellular level
      within a given tissue, and protein amounts in the cytoplasm and nucleus varied
      widely, suggesting a highly regulated and intermittent function for p97. This
      study provides the basis for a detailed analysis of the complex regulation of p97
      and the reagents required for assessing its functional significance using
      targeted gene manipulation in the mouse.
FAU - Muller, J M
AU  - Muller JM
AD  - Cell Biology Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields,
      London WC2A 3PX, UK.
FAU - Meyer, H H
AU  - Meyer HH
FAU - Ruhrberg, C
AU  - Ruhrberg C
FAU - Stamp, G W
AU  - Stamp GW
FAU - Warren, G
AU  - Warren G
FAU - Shima, D T
AU  - Shima DT
LA  - eng
SI  - GENBANK/AF122047
SI  - GENBANK/AF122048
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.6 (Valosin Containing Protein)
SB  - IM
MH  - Adenosine Triphosphatases
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins/*genetics/*physiology
MH  - Cell Differentiation
MH  - Cell Division
MH  - Drosophila melanogaster
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - *Pseudogenes
MH  - Structure-Activity Relationship
MH  - *Transcription, Genetic
MH  - Valosin Containing Protein
MH  - Xenopus laevis
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10154 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10154-62. doi: 10.1074/jbc.274.15.10154.