PMID- 10187784
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 15
DP  - 1999 Apr 9
TI  - Molecular uncoupling of fractalkine-mediated cell adhesion and signal
      transduction. Rapid flow arrest of CX3CR1-expressing cells is independent of
      G-protein activation.
PG  - 10053-8
AB  - Fractalkine is a novel multidomain protein expressed on the surface of activated 
      endothelial cells. Cells expressing the chemokine receptor CX3CR1 adhere to
      fractalkine with high affinity, but it is not known if adherence requires
      G-protein activation and signal transduction. To investigate the cell adhesion
      properties of fractalkine, we created mutated forms of CX3CR1 that have little or
      no ability to transduce intracellular signals. Cells expressing
      signaling-incompetent forms of CX3CR1 bound rapidly and with high affinity to
      immobilized fractalkine in both static and flow assays. Video microscopy revealed
      that CX3CR1-expressing cells bound more rapidly to fractalkine than to VCAM-1 (60
      versus 190 ms). Unlike VCAM-1, fractalkine did not mediate cell rolling, and
      after capture on fractalkine, cells did not dislodge. Finally, soluble
      fractalkine induced intracellular calcium fluxes and chemotaxis, but it did not
      activate integrins. Taken together these data provide strong evidence that
      CX3CR1, a seven-transmembrane domain receptor, mediates robust cell adhesion to
      fractalkine in the absence of G-protein activation and suggest a novel role for
      this receptor as an adhesion molecule.
FAU - Haskell, C A
AU  - Haskell CA
AD  - Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-9100, USA.
FAU - Cleary, M D
AU  - Cleary MD
FAU - Charo, I F
AU  - Charo IF
LA  - eng
GR  - HL52773/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (CX3CL1 protein, human)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines, CX3C)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cx3cl1 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, HIV)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - CX3C Chemokine Receptor 1
MH  - *Cell Adhesion
MH  - Cell Line
MH  - Chemokine CX3CL1
MH  - *Chemokines, CX3C
MH  - Chemokines, CXC/*metabolism
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Receptors, Cytokine/genetics/*metabolism
MH  - Receptors, HIV/genetics/*metabolism
MH  - *Signal Transduction
MH  - Solubility
MH  - Transfection
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - 10.1074/jbc.274.15.10053 [doi]
AID - S0021-9258(19)73682-5 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 9;274(15):10053-8. doi: 10.1074/jbc.274.15.10053.